Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia.
Eur J Clin Microbiol Infect Dis. 2020 Jan;39(1):19-30. doi: 10.1007/s10096-019-03680-2. Epub 2019 Aug 20.
Infection by Toxoplasma gondii is prevalent worldwide. The parasite can infect a broad spectrum of vertebrate hosts, but infection of fetuses and immunocompromised patients is of particular concern. Easy-to-perform, robust, and highly sensitive and specific methods to detect Toxoplasma infection are important for the treatment and management of patients. Rapid diagnostic methods that do not sacrifice the accuracy of the assay and give reproducible results in a short time are highly desirable. In this context, rapid diagnostic tests (RDTs), especially with point-of-care (POC) features, are promising diagnostic methods in clinical microbiology laboratories, especially in areas with minimal laboratory facilities. More advanced methods using microfluidics and sensor technology will be the future trend. In this review, we discuss serological and molecular-based rapid diagnostic tests for detecting Toxoplasma infection in humans as well as animals.
弓形虫感染在全球范围内普遍存在。该寄生虫可以感染广泛的脊椎动物宿主，但胎儿和免疫功能低下患者的感染尤其令人关注。能够简便、可靠、高度敏感和特异检测弓形虫感染的方法对于患者的治疗和管理非常重要。在不牺牲检测准确性的情况下，在短时间内获得可重复结果的快速诊断方法是非常需要的。在这种情况下，快速诊断检测（RDT），特别是具有即时检测（POC）功能的 RDT，是临床微生物学实验室中很有前途的诊断方法，尤其是在实验室设施有限的地区。使用微流控和传感器技术的更先进方法将是未来的趋势。在这篇综述中，我们讨论了用于检测人类和动物弓形虫感染的血清学和基于分子的快速诊断检测。
