Department of Plant Physiology, University of Gdańsk, Gdynia, Poland.
Bull Environ Contam Toxicol. 2011 Sep;87(3):226-30. doi: 10.1007/s00128-011-0339-3. Epub 2011 Jun 18.
The objective of this study was to examine the influence of different stressors, including cadmium (heavy metal), anthracene (polycyclic aromatic hydrocarbon-PAH) and chloridazon (herbicide), on population growth and biosynthesis of cytoplasmic HSP70 in Lemna minor (duckweed) in short (4 h)- and long (7 days)-term tests. A heat shock response was confirmed in Lemna exposed to high temperature: 35, 37.5, 40, or 42.5°C in short-term (4 h) treatments. The chemicals tested stimulated the biosynthesis of the cytoplasmic HSP70 protein in a concentration-dependent way (0.5-5 μM), higher in fronds exposed to lower doses of stressors. Additionally, production of HSP70 was greater after 4 h of incubation than after 7 days. The results suggest that HSP70 could be applied as a non-specific and sensitive detector of stress induced by different chemicals at concentrations below those that produce the type of response observed in classical cytotoxicity tests, such as growth inhibition.
本研究旨在探讨不同应激源（包括镉（重金属）、蒽（多环芳烃-PAH）和氯哒嗪（除草剂））对小浮萍（浮萍）种群生长和细胞质 HSP70 生物合成的影响，分别进行短期（4 小时）和长期（7 天）试验。热激反应在暴露于高温（35、37.5、40 或 42.5°C）的浮萍中得到证实。在短期（4 小时）处理中，测试的化学物质以浓度依赖的方式刺激细胞质 HSP70 蛋白的生物合成（0.5-5 μM），在暴露于较低剂量应激源的叶片中更高。此外，孵育 4 小时后 HSP70 的产生量大于孵育 7 天后的产生量。结果表明，HSP70 可以作为一种非特异性和敏感的检测器，用于检测不同化学物质在低于引起经典细胞毒性试验（如生长抑制）中观察到的反应类型的浓度下引起的应激。
