Skóra Magdalena, Macura Anna B
Department of Mycology, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Kraków, Poland.
Wiad Parazytol. 2011;57(2):111-6.
The genus Scopulariopsis is a common soil saprotroph and has been isolated from air, organic waste and also from plant, animal and human tissues. Scopulariopsis has mainly been associated in humans with superficial mycoses, but it has also been described as the cause of subcutaneous and invasive infections. The most common aetiological agent of infections in humans is Scopulariopsis brevicaulis. This species has been reported to be resistant in vitro to broad-spectrum antifungal agents available today. The aim of the study was to establish in vitro antifungal susceptibility of 35 S. brevicaulis strains against amphotericin B (AMB), flucytosine (FC), caspofungin (CAS), terbinafine (TER), ciclopirox (CIC), voriconazole (VOR), clotrimazole (CTR), miconazole (MCZ), econazole (ECO), ketoconazole (KET), itraconazole (ITR), and fluconazole (FLU). Antifungal susceptibility tests were evaluated by an agar diffusion method (Neo-Sensitabs, Rosco, Denmark). AMB, FC, CAS, ITR and FLU showed no antifungal activity against S. brevicaulis. TER, CIC, CTR, KET, VOR, ECO, and MCZ revealed inhibitory activity for S. brevicaulis, but it varied for each of the drugs. The best antifungal effect was observed for TER and CIC. All isolates had large inhibition zones for TER and CIC. CTR was also inhibitory for all tested S. brevicaulis isolates, but the diameters of inhibition zones were smaller than for TER and CIC. Nearly 89% isolates showed inhibition zones for KET and the mean diameter of the inhibition zone was comparable to CTR. The least antifungal activity exhibited VQR, ECO and MCZ. Because of the multiresistance of S. brevicaulis, infections due to this species may not respond to particular antifungal treatment and other therapeutic approaches should be considered, e.g., combined therapy and/or surgery.
帚霉属是一种常见的土壤腐生菌，已从空气、有机废物以及植物、动物和人体组织中分离出来。帚霉在人类中主要与浅表真菌病相关，但也被描述为皮下和侵袭性感染的病因。人类感染最常见的病原体是短帚霉。据报道，该菌种在体外对目前可用的广谱抗真菌药物具有抗性。本研究的目的是确定35株短帚霉菌株对两性霉素B（AMB）、氟胞嘧啶（FC）、卡泊芬净（CAS）、特比萘芬（TER）、环吡酮（CIC）、伏立康唑（VOR）、克霉唑（CTR）、咪康唑（MCZ）、益康唑（ECO）、酮康唑（KET）、伊曲康唑（ITR）和氟康唑（FLU）的体外抗真菌敏感性。采用琼脂扩散法（丹麦Rosco公司的Neo - Sensitabs）评估抗真菌药敏试验。AMB、FC、CAS、ITR和FLU对短帚霉无抗真菌活性。TER、CIC、CTR、KET、VOR、ECO和MCZ对短帚霉显示出抑制活性，但每种药物的活性有所不同。观察到TER和CIC的抗真菌效果最佳。所有分离株对TER和CIC都有较大的抑菌圈。CTR对所有测试的短帚霉分离株也有抑制作用，但抑菌圈直径小于TER和CIC。近89%的分离株对KET有抑菌圈，抑菌圈平均直径与CTR相当。VOR、ECO和MCZ表现出的抗真菌活性最低。由于短帚霉具有多重耐药性，该菌种引起的感染可能对特定的抗真菌治疗无反应，应考虑其他治疗方法，如联合治疗和/或手术。
