Wu Mengxi, Ma Zhiteng, Tian Zhenhua, Rich Joseph T, He Xin, Xia Jianping, He Ye, Yang Kaichun, Yang Shujie, Leong Kam W, Lee Luke P, Huang Tony Jun
School of Mechanical Engineering, Dalian University of Technology, Dalian, 116086, Liaoning, China.
Thomas Lord Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.
Microsyst Nanoeng. 2024 Nov 19;10(1):170. doi: 10.1038/s41378-024-00759-5.
Advanced biofabrication techniques can create tissue-like constructs that can be applied for reconstructive surgery or as in vitro three-dimensional (3D) models for disease modeling and drug screening. While various biofabrication techniques have recently been widely reviewed in the literature, acoustics-based technologies still need to be explored. The rapidly increasing number of publications in the past two decades exploring the application of acoustic technologies highlights the tremendous potential of these technologies. In this review, we contend that acoustics-based methods can address many limitations inherent in other biofabrication techniques due to their unique advantages: noncontact manipulation, biocompatibility, deep tissue penetrability, versatility, precision in-scaffold control, high-throughput capabilities, and the ability to assemble multilayered structures. We discuss the mechanisms by which acoustics directly dictate cell assembly across various biostructures and examine how the advent of novel acoustic technologies, along with their integration with traditional methods, offers innovative solutions for enhancing the functionality of organoids. Acoustic technologies are poised to address fundamental challenges in biofabrication and tissue engineering and show promise for advancing the field in the coming years.
先进的生物制造技术可以创建类似组织的构建体，可应用于重建手术或用作疾病建模和药物筛选的体外三维（3D）模型。虽然最近文献中对各种生物制造技术进行了广泛综述，但基于声学的技术仍有待探索。在过去二十年中，探索声学技术应用的出版物数量迅速增加，凸显了这些技术的巨大潜力。在本综述中，我们认为基于声学的方法因其独特优势可以解决其他生物制造技术固有的许多局限性：非接触操作、生物相容性、深层组织穿透性、多功能性、支架内控制精度、高通量能力以及组装多层结构的能力。我们讨论了声学直接决定跨各种生物结构的细胞组装的机制，并研究了新型声学技术的出现及其与传统方法的整合如何为增强类器官的功能提供创新解决方案。声学技术有望解决生物制造和组织工程中的基本挑战，并在未来几年推动该领域的发展。
