DiGiancamillo Alessia, Domeneghini Cinzia, Gibelli Daniele, Cattaneo Cristina
Dipartimento di Scienze e Tecnologie Veterinarie per la Sicurezza Alimentare, Università degli Studi di Milano, Italy.
Leg Med (Tokyo). 2011 Sep;13(5):268-71. doi: 10.1016/j.legalmed.2011.05.005. Epub 2011 Jun 16.
Identification of diatoms from human or animal tissue may be affected by factors concerning techniques of extraction and analysis which cause fragmentation or loss of diatoms. In this brief technical note a novel efficient method of extraction of diatoms from animal tissues is presented. The authors have tested an H(2)O(2) plus HCl and HCl method to detect diatoms in several tissues from pig immersed in water rich in diatoms; they have also compared this method to other methods of extraction including enzymatic ones. The experiment showed that all siliceous frustules of fresh water diatoms are resistant to the treatment proposed and are still recognizable after the digestion. Among the different tests, treatment by 20% HCl (T1) proved to be more simple and time-saving, and seems to be a valuable diagnostic tool for routine forensic procedures in the extraction and detection of diatoms. The positive results obtained in all cases should lead to further studies in order to evaluate the reliability of the proposed methods also from a quantitative point of view.
从人或动物组织中鉴定硅藻可能会受到提取和分析技术相关因素的影响，这些因素会导致硅藻破碎或损失。在这篇简短的技术说明中，介绍了一种从动物组织中提取硅藻的新型高效方法。作者测试了过氧化氢加盐酸和盐酸法，以检测浸泡在富含硅藻的水中的猪的几种组织中的硅藻；他们还将该方法与包括酶法在内的其他提取方法进行了比较。实验表明，淡水硅藻的所有硅质壳对所提出的处理具有抗性，消化后仍可识别。在不同的测试中，20%盐酸处理（T1）被证明更简单、省时，似乎是硅藻提取和检测常规法医程序中有价值的诊断工具。在所有情况下获得的阳性结果应促使进一步研究，以便从定量角度评估所提出方法的可靠性。
