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T4 样噬菌体中保守的regA基因和可变的orf43.1基因的序列分析。

Sequence analysis of conserved regA and variable orf43.1 genes in T4-like bacteriophages.

作者信息

Miller E S, Jozwik C E

机构信息

Department of Microbiology, North Carolina State University, Raleigh 27695-7615.

出版信息

J Bacteriol. 1990 Sep;172(9):5180-6. doi: 10.1128/jb.172.9.5180-5186.1990.

DOI:10.1128/jb.172.9.5180-5186.1990
PMID:2168375
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC213179/
Abstract

Bacteriophage T4 RegA protein is a translational repressor of several phage mRNAs. In the T4-related phages examined, regA nucleotide sequences are highly conserved and the inferred amino acid sequences are identical. The exceptional phage, RB69, did not produce a RegA protein reproducibly identifiable by Western blots (immunoblots) nor did it produce mRNA that hybridized to T4 regA primers. Nucleotide sequences of either 223 or 250 base pairs were identified immediately 3' to regA in RB18 and RB51 that were absent in T-even phages. Open reading frames in these regions, designated orf43.1RB18 and orf43.1RB51, potentially encode related proteins of 8.5 and 9.2 kilodaltons, respectively. orf43.1 sequences, detected in 13 of 27 RB bacteriophage chromosomes analyzed by polymerase chain reaction, are either RB18- or RB51-like and have flanking repeat sequences that may promote orf43.1 deletion.

摘要

噬菌体T4 RegA蛋白是几种噬菌体mRNA的翻译阻遏物。在所检测的与T4相关的噬菌体中，regA核苷酸序列高度保守，推测的氨基酸序列相同。特殊的噬菌体RB69，通过蛋白质免疫印迹法（免疫印迹法）无法重复鉴定出RegA蛋白，也未产生与T4 regA引物杂交的mRNA。在RB18和RB51中，在regA 3'端紧邻处鉴定出223或250个碱基对的核苷酸序列，而偶数噬菌体中不存在这些序列。这些区域的开放阅读框，分别命名为orf43.1RB18和orf43.1RB51，可能分别编码8.5和9.2千道尔顿的相关蛋白。通过聚合酶链反应分析的27条RB噬菌体染色体中有13条检测到orf43.1序列，这些序列要么类似RB18，要么类似RB51，并且具有侧翼重复序列，可能促进orf43.1的缺失。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/add2/213179/3e71d128fe2e/jbacter00123-0453-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/add2/213179/3ab6f7c2f5e9/jbacter00123-0450-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/add2/213179/51528ad3978f/jbacter00123-0452-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/add2/213179/3e71d128fe2e/jbacter00123-0453-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/add2/213179/3ab6f7c2f5e9/jbacter00123-0450-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/add2/213179/51528ad3978f/jbacter00123-0452-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/add2/213179/3e71d128fe2e/jbacter00123-0453-a.jpg

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