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具有极性galE突变的大肠杆菌K-12菌株将半乳糖高效掺入脂多糖。

Efficient incorporation of galactose into lipopolysaccharide by Escherichia coli K-12 strains with polar galE mutations.

作者信息

Schnaitman C A, Austin E A

机构信息

Department of Microbiology, University of Virginia Medical School, Charlottesville 22908.

出版信息

J Bacteriol. 1990 Sep;172(9):5511-3. doi: 10.1128/jb.172.9.5511-5513.1990.

DOI:10.1128/jb.172.9.5511-5513.1990
PMID:2168387
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC213224/
Abstract

Efficient incorporation of exogenous galactose into lipopolysaccharide was observed in a strain with a galE::Tn10 insertion and a strain with a deletion of galOPE. No incorporation was observed in a strain with a longer (galETK) deletion, indicating that incorporation into the galE mutants was due to a low level of expression of galTK from internal or fortuitous promoters. These galE mutants provided a convenient and specific way to radiolabel lipopolysaccharide.

摘要

在一个带有galE::Tn10插入的菌株和一个缺失galOPE的菌株中，观察到外源半乳糖有效地掺入到脂多糖中。在一个具有更长（galETK）缺失的菌株中未观察到掺入，这表明掺入到galE突变体中是由于来自内部或偶然启动子的galTK低水平表达所致。这些galE突变体提供了一种方便且特异的方法来放射性标记脂多糖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f78/213224/23b36a5133d6/jbacter00123-0782-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f78/213224/8b0ae98a1d8d/jbacter00123-0782-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f78/213224/23b36a5133d6/jbacter00123-0782-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f78/213224/8b0ae98a1d8d/jbacter00123-0782-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5f78/213224/23b36a5133d6/jbacter00123-0782-b.jpg

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