Parasitological Institute, Slovak Academy of Sciences, Košice, Slovak Republic.
Vet Microbiol. 2011 Dec 15;153(3-4):293-8. doi: 10.1016/j.vetmic.2011.05.044. Epub 2011 Jun 1.
Anaplasmoses are common tick-borne zoonotic bacterial diseases of livestock and free-living ungulates from the genus Anaplasma that are recently emerging in Central Europe. The main aim of this study was to analyze the prevalence and genetic variability of Anaplasma phagocytophilum and Anaplasma ovis in small ruminants and questing ticks from six different sites in Slovakia and the Czech Republic using the PCR of the msp4 gene followed by the sequence analysis. At two farms from southeastern Slovakia, 66.1% small ruminants were infected with A. ovis in contrast to one positive animal from both sites in northern Slovakia. It was represented by two different genotypes. A. phagocytophilum was present in all tested flocks with the infection prevalence ranging from 0.9% to 5.7%. None of the tested questing ticks carried A. ovis. A. phagocytophilum was detected in 1.1% and 7.8% of questing Ixodes ricinus ticks collected around the farms located in southeastern and northern Slovakia, respectively. A. phagocytophilum revealed higher intraspecific diversity than A. ovis.
类立克次体病是一种常见的蜱传人畜共患细菌性疾病，病原体为绵羊泰勒虫属和边缘无浆体属的细菌，最近在中欧地区流行。本研究的主要目的是使用 msp4 基因 PCR 结合序列分析，分析来自斯洛伐克和捷克共和国六个不同地点的小反刍动物和游离蜱中的绵羊泰勒虫和边缘无浆体的流行率和遗传变异。在来自斯洛伐克东南部的两个农场，66.1%的小反刍动物感染了边缘无浆体，而在来自斯洛伐克北部的两个地点，只有一个动物呈阳性。它由两种不同的基因型代表。在所有检测的羊群中均存在绵羊泰勒虫，感染率为 0.9%至 5.7%。未在检测的游离硬蜱中发现边缘无浆体。在来自斯洛伐克东南部和北部农场周围采集的游离硬蜱中，分别检测到 1.1%和 7.8%的绵羊泰勒虫。绵羊泰勒虫的种内多样性高于边缘无浆体。
