Karatepe Mustafa, Aktaş Münir, Karatepe Bilge, Özübek Sezayi
Faculty of Science, Department of Biotechnology, Niğde Ömer Halisdemir University, 51240, Niğde, Türkiye.
Faculty of Veterinary, Department of Parasitology, Fırat University, 23200, Elazığ, Türkiye.
Acta Parasitol. 2025 May 27;70(3):114. doi: 10.1007/s11686-025-01056-5.
This study was carried out to investigate Anaplasma important for veterinary and public health in sheep and goats in Niğde province in Türkiye by using molecular methods.
Blood samples were taken from randomly selected 690 animals (520 sheep and 170 goats), which were between 1 and 10 years old and from different study sites in Niğde by using the vacutainer tubes containing EDTA. After the genomic DNA extractions samples, the Anaplasma spp. 16S rRNA genes were amplified by PCR. Species-specific polymerase chain reaction (PCR) assays were performed on positive samples for the presence of A. bovis, A. capra, A. ovis, A. platys-like, and A. phagocytophilum. At the same time, the animals were tested for ixodid tick infestation and collected ticks were examined for identification under the stereo-microscope.
The results of PCR analysis show that the overall A. ovis prevalence was 63.3% (437/690) in small ruminants sampled. A total of 361 sheep (69.4%) and 76 goats (44.7%) were found to be infected with A. ovis, whereas no positivity was detected for A. bovis, A. capra, A. platys-like, and A. phagocytophilum. Anaplasma ovis positivity was observed at the highest percent in May (%74.6) while the lowest in June (%52.4). In total, 1361 ticks (579♀, 782♂) were collected from sheep and goats in Niğde. Ticks were identified as Rhipicephalus bursa (383, 28.1%), R. turanicus (607, 44.6%), Hyalomma marginatum (7, 0.5%), Hy. excavatum (247, 18.1%), Hy. anatolicum (23, 1.7%), Haemophsalis parva (21, 1.5%), Hae. punctata (7, 0.5%), Hae. sulcata (40, 2.9%) and Dermacentor marginatus (26, 1.9%).
The present study reports a high prevalence of A. ovis 63.3% (437/690) in sheep and goats in Niğde province.
本研究旨在通过分子方法调查土耳其尼德省绵羊和山羊体内对兽医和公共卫生具有重要意义的无形体。
使用含有乙二胺四乙酸（EDTA）的真空采血管，从尼德省不同研究地点随机选取690只年龄在1至10岁之间的动物（520只绵羊和170只山羊）采集血样。提取基因组DNA样本后，通过聚合酶链反应（PCR）扩增无形体属16S rRNA基因。对阳性样本进行种特异性聚合酶链反应（PCR）检测，以确定是否存在牛无形体、山羊无形体、绵羊无形体、类血小板无形体和嗜吞噬细胞无形体。同时，对动物进行硬蜱感染检测，并在体视显微镜下检查采集到的蜱以进行鉴定。
PCR分析结果显示，在所采样的小反刍动物中，绵羊无形体的总体感染率为63.3%（437/690）。共发现361只绵羊（69.4%）和76只山羊（44.7%）感染了绵羊无形体，而未检测到牛无形体、山羊无形体、类血小板无形体和嗜吞噬细胞无形体呈阳性。绵羊无形体阳性率在5月份最高（74.6%），6月份最低（52.4%）。总共从尼德省的绵羊和山羊身上采集到1361只蜱（579只雌蜱，782只雄蜱）。蜱被鉴定为柏氏扇头蜱（383只，28.1%）、图兰扇头蜱（607只，44.6%）、缘璃眼蜱（7只，0.5%）、刻点璃眼蜱（247只，18.1%）、安纳托利亚璃眼蜱（23只，1.7%）、微小血蜱（21只，1.5%）、钝缘血蜱（7只，0.5%）、苏氏血蜱（40只，2.9%）和边缘革蜱（26只，1.9%）。
本研究报告了土耳其尼德省绵羊和山羊中绵羊无形体的高感染率，为63.3%（437/690）。
