钙对经地塞米松和二甲基亚砜处理的细胞中巨细胞病毒检测的增强作用。
Enhancement by calcium of the detection of cytomegalovirus in cells treated with dexamethasone and dimethyl sulfoxide.
作者信息
West P G, Baker W W
机构信息
SmithKline Beecham Clinical Laboratories, Norristown, Pennsylvania 19403.
出版信息
J Clin Microbiol. 1990 Aug;28(8):1708-10. doi: 10.1128/jcm.28.8.1708-1710.1990.
MRC-5 cultures treated with dexamethasone, dimethyl sulfoxide, and calcium were compared with untreated cultures and cultures treated with dexamethasone and dimethyl sulfoxide for the detection of human cytomegalovirus by the shell vial method. The addition of calcium resulted in significantly increased growth of human cytomegalovirus AD169. Protein studies and cell counts showed renewed cell proliferation in the treated cultures. When low-titer clinical specimens were retested, 27.5% of all positives revealed were found only on the vials treated with calcium. In a group of freshly tested specimens, the calcium-treated group accounted for 20% of the positives.
将用 dexamethasone、二甲基亚砜和钙处理的 MRC - 5 培养物与未处理的培养物以及用 dexamethasone 和二甲基亚砜处理的培养物进行比较，通过空斑试验检测人巨细胞病毒。添加钙导致人巨细胞病毒 AD169 的生长显著增加。蛋白质研究和细胞计数显示处理后的培养物中有新的细胞增殖。当对低滴度临床标本进行重新检测时，所有检测出的阳性标本中，有 27.5%仅在添加钙处理的小瓶中被发现。在一组新检测的标本中，添加钙处理的组占阳性结果的 20%。
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