增强剂对临床标本中巨细胞病毒检测的影响。
Effects of enhancing agents on detection of cytomegalovirus in clinical specimens.
作者信息
St George K, Rinaldo C R
机构信息
Department of Pathology, University of Pittsburgh Medical Center, Pennsylvania 15213.
出版信息
J Clin Microbiol. 1994 Aug;32(8):2024-7. doi: 10.1128/jcm.32.8.2024-2027.1994.
Abstract
Dimethyl sulfoxide, dexamethasone, and calcium were tested in combination for their enhancing effects on cytomegalovirus detection in shell vial cultures on 1,579 clinical specimens obtained primarily from adult solid-organ transplant recipients. Fluorescent-focus counts were elevated for the cytomegalovirus-positive urine specimens (P < 0.01) and throat washings (P < 0.05) but not for the tissue biopsy or blood samples. Epidermal growth factor also increased focus counts but provided no additional benefit when used in combination with the other agents. The triple-combination treatment did not increase the number of positive specimens identified.
摘要
对1579份主要取自成年实体器官移植受者的临床标本，测试了二甲亚砜、地塞米松和钙联合使用对壳瓶培养中巨细胞病毒检测的增强作用。巨细胞病毒阳性尿液标本（P < 0.01）和咽洗液标本（P < 0.05）的荧光灶计数升高，但组织活检标本和血液样本未升高。表皮生长因子也增加了灶计数，但与其他试剂联合使用时未提供额外益处。三联组合治疗未增加鉴定出的阳性标本数量。
相似文献
1
Effects of enhancing agents on detection of cytomegalovirus in clinical specimens.增强剂对临床标本中巨细胞病毒检测的影响。
J Clin Microbiol. 1994 Aug;32(8):2024-7. doi: 10.1128/jcm.32.8.2024-2027.1994.
2
Enhancement by calcium of the detection of cytomegalovirus in cells treated with dexamethasone and dimethyl sulfoxide.钙对经地塞米松和二甲基亚砜处理的细胞中巨细胞病毒检测的增强作用。
J Clin Microbiol. 1990 Aug;28(8):1708-10. doi: 10.1128/jcm.28.8.1708-1710.1990.
3
Enhanced detection of cytomegalovirus in confluent MRC-5 cells treated with dexamethasone and dimethyl sulfoxide.
J Clin Microbiol. 1988 Dec;26(12):2510-4. doi: 10.1128/jcm.26.12.2510-2514.1988.
4
Effect of treatment of shell vial cell cultures with dimethyl sulfoxide and dexamethasone for detection of cytomegalovirus.用二甲基亚砜和地塞米松处理空斑试验细胞培养物对检测巨细胞病毒的影响。
J Clin Microbiol. 1988 Jun;26(6):1091-3. doi: 10.1128/jcm.26.6.1091-1093.1988.
5
Effect of treatment of shell vial cell cultures with dimethyl sulfoxide and dexamethasone and age of MRC-5 monolayers for detection of cytomegalovirus.用二甲基亚砜和地塞米松处理空斑试验细胞培养物以及MRC - 5单层细胞的年龄对巨细胞病毒检测的影响。
Diagn Microbiol Infect Dis. 1990 Jan-Feb;13(1):41-4. doi: 10.1016/0732-8893(90)90052-w.
6
Improving permissive infection of human cytomegalovirus in cell culture.提高人巨细胞病毒在细胞培养中的允许性感染。
Arch Virol. 2000;145(11):2431-8. doi: 10.1007/s007050070032.
7
Use of a modified shell vial technique to quantitate cytomegalovirus viremia in a population of solid-organ transplant recipients.使用改良的空斑试验技术对实体器官移植受者群体中的巨细胞病毒血症进行定量分析。
J Clin Microbiol. 1992 Oct;30(10):2620-4. doi: 10.1128/jcm.30.10.2620-2624.1992.
8
Comparison of sensitivities of three commercial MRC-5 cell lines grown in shell vials to cytomegalovirus and responses to enhancing agents.三种在细胞培养小瓶中生长的商业化MRC - 5细胞系对巨细胞病毒的敏感性比较以及对增强剂的反应。
J Clin Microbiol. 1992 Mar;30(3):557-60. doi: 10.1128/jcm.30.3.557-560.1992.
9
Simultaneous culture for adenovirus, cytomegalovirus, and herpes simplex virus in same shell vial by using three-color fluorescence.通过三色荧光在同一空斑小瓶中同时培养腺病毒、巨细胞病毒和单纯疱疹病毒。
J Clin Microbiol. 1994 Sep;32(9):2289-90. doi: 10.1128/jcm.32.9.2289-2290.1994.
10
Enhanced detection of cytomegalovirus in shell vial culture following MRC-5 monolayer pretreatment with glucocorticoids.用糖皮质激素预处理MRC-5单层细胞后，在空斑试验培养中增强巨细胞病毒的检测。
Zentralbl Bakteriol. 1992 Jun;277(1):90-9. doi: 10.1016/s0934-8840(11)80876-5.
引用本文的文献
1
Glucocorticosteroids trigger reactivation of human cytomegalovirus from latently infected myeloid cells and increase the risk for HCMV infection in D+R+ liver transplant patients.糖皮质激素可触发人巨细胞病毒从潜伏感染的髓系细胞中重新激活，并增加D+R+肝移植患者感染HCMV的风险。
J Gen Virol. 2015 Jan;96(Pt 1):131-143. doi: 10.1099/vir.0.069872-0. Epub 2014 Oct 13.
2
Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections.呼吸道病毒感染快速检测的多重实时 PCR 试剂盒的临床评估。
J Med Virol. 2012 Jan;84(1):162-9. doi: 10.1002/jmv.22186. Epub 2011 Nov 3.
本文引用的文献
1
Calcium pretreatment induces the decrease in epidermal growth factor binding through the activation of transglutaminase in isolated liver membrane.钙预处理通过激活离体肝细胞膜中的转谷氨酰胺酶诱导表皮生长因子结合减少。
Arch Biochem Biophys. 1993 Jun;303(2):421-8. doi: 10.1006/abbi.1993.1304.
2
Rapid diagnosis of cytomegalovirus infection in immunocompromised patients by detection of early antigen fluorescent foci.通过检测早期抗原荧光病灶快速诊断免疫功能低下患者的巨细胞病毒感染。
Lancet. 1984 Dec 1;2(8414):1242-5. doi: 10.1016/s0140-6736(84)92797-1.
3
Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen.通过低速离心和使用针对早期抗原的单克隆抗体，快速检测接种尿液标本的MRC-5细胞中的巨细胞病毒。
J Clin Microbiol. 1984 Jun;19(6):917-9. doi: 10.1128/jcm.19.6.917-919.1984.
4
Enhanced detection of cytomegalovirus in confluent MRC-5 cells treated with dexamethasone and dimethyl sulfoxide.
J Clin Microbiol. 1988 Dec;26(12):2510-4. doi: 10.1128/jcm.26.12.2510-2514.1988.
5
The effect of dexamethasone on the detection of cytomegalovirus in tissue culture and by immunofluorescence.地塞米松对组织培养中巨细胞病毒检测及免疫荧光检测的影响。
J Virol Methods. 1988 Dec;22(2-3):319-28. doi: 10.1016/0166-0934(88)90114-0.
6
Immunological detection of cytomegalovirus early antigen on monolayers inoculated with urine specimens by centrifugation and cultured for 6 days as alternative to conventional virus isolation.通过离心接种尿液标本的单层细胞并培养6天，以免疫方法检测巨细胞病毒早期抗原，作为传统病毒分离方法的替代方法。
J Clin Microbiol. 1988 Jul;26(7):1313-5. doi: 10.1128/jcm.26.7.1313-1315.1988.
7
Effect of treatment of shell vial cell cultures with dimethyl sulfoxide and dexamethasone for detection of cytomegalovirus.用二甲基亚砜和地塞米松处理空斑试验细胞培养物对检测巨细胞病毒的影响。
J Clin Microbiol. 1988 Jun;26(6):1091-3. doi: 10.1128/jcm.26.6.1091-1093.1988.
8
Comparison of two rapid culture methods for detection of cytomegalovirus in clinical specimens.两种快速培养方法用于检测临床标本中巨细胞病毒的比较。
J Clin Microbiol. 1987 Dec;25(12):2445-6. doi: 10.1128/jcm.25.12.2445-2446.1987.
9
Rapid detection of cytomegalovirus by tissue culture, centrifugation, and immunofluorescence with a monoclonal antibody to an early nuclear antigen.通过组织培养、离心以及使用针对早期核抗原的单克隆抗体进行免疫荧光检测来快速检测巨细胞病毒。
J Virol Methods. 1987 Jul;16(4):327-38. doi: 10.1016/0166-0934(87)90018-8.
10
Rapid diagnosis of invasive cytomegalovirus infection by examination of tissue specimens in centrifugation culture.通过离心培养检查组织标本快速诊断巨细胞病毒侵袭性感染。
Am J Clin Pathol. 1987 Sep;88(3):354-8. doi: 10.1093/ajcp/88.3.354.
Zotero 插件