Espy M J, Wold A D, Ilstrup D M, Smith T F
Section of Clinical Microbiology, Mayo Clinic and Foundation, Rochester, Minnesota 55905.
J Clin Microbiol. 1988 Jun;26(6):1091-3. doi: 10.1128/jcm.26.6.1091-1093.1988.
Urine specimens submitted for the diagnosis of cytomegalovirus infection were inoculated into shell vials that had been pretreated with a combination of dimethyl sulfoxide (DMSO) and dexamethasone (DEX). The results were compared with those for inoculated shell vials which had received no drug treatment. Of 664 specimens, 100 (15%) were positive for cytomegalovirus. Of the 100 strains of cytomegalovirus, 88 (88%) were detected in both DMSO-DEX-treated and untreated shell vials. Of the remaining 12 positive specimens, 6 were detected with untreated shell vials exclusively and 6 were detected with DMSO-DEX-treated shell vials alone (not significant by the sign test). The median number of fluorescent foci was not significantly higher in DMSO-DEX-treated shell vials compared with that in untreated cultures (Wilcoxon signed-rank test; P = 0.1). DMSO-DEX-treated monolayers did not enhance the sensitivity detection of cytomegalovirus in shell vial cell cultures.
将用于诊断巨细胞病毒感染的尿液标本接种到已用二甲基亚砜（DMSO）和地塞米松（DEX）组合预处理的空斑试验瓶中。将结果与未接受药物处理的接种空斑试验瓶的结果进行比较。在664份标本中，100份（15%）巨细胞病毒呈阳性。在100株巨细胞病毒中，88株（88%）在经DMSO-DEX处理和未处理的空斑试验瓶中均被检测到。在其余12份阳性标本中，6份仅在未处理的空斑试验瓶中被检测到，6份仅在经DMSO-DEX处理的空斑试验瓶中被检测到（符号检验无显著性差异）。与未处理的培养物相比，经DMSO-DEX处理的空斑试验瓶中荧光灶的中位数没有显著更高（Wilcoxon符号秩检验；P = 0.1）。经DMSO-DEX处理的单层细胞并没有提高空斑试验瓶细胞培养中巨细胞病毒的检测灵敏度。
