Ciarlet Max, Hyser Joseph M, Estes Mary K
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA.
Virus Genes. 2002 Mar;24(2):107-18. doi: 10.1023/a:1014512314545.
The bovine rotavirus (BRV) WC3 serves as the background strain in the development of a multivalent reassortant vaccine against rotavirus gastroenteritis in infants. The genes encoding the outer capsid spike protein VP4, the inner capsid protein VP6, the outer capsid glycoprotein VP7, and the viral enterotoxin NSP4 of BRV WC3 were sequenced. Comparative analysis of the deduced amino acids of the sequenced genes indicated that the BRV WC3 strain shares a high degree of amino acid identity with serotype P7 VP4 (93-96%), serotype G6 VP7 (91-97%), subgroup (SG) I VP6 (96-99%), and NSP4 genogroup A (96-98%) BRV strains. Our results confirm and extend previous studies which suggested that the VP4 of BRV WC3 was closely related to that of the P7 prototype, BRV UK. In addition, the VP6 and VP7 of BRV WC3 were very similar to the VP6 and VP7 of both SG I and G6 BRV NCDV and UK strains. However, the NSP4 of BRV WC3 was more closely related to that BRV NCDV, the P6 prototype, than to that of BRV UK.
牛轮状病毒（BRV）WC3是开发针对婴儿轮状病毒肠胃炎的多价重配疫苗的背景毒株。对编码BRV WC3外 capsid 刺突蛋白VP4、内 capsid 蛋白VP6、外 capsid 糖蛋白VP7和病毒肠毒素NSP4的基因进行了测序。对测序基因推导的氨基酸进行的比较分析表明，BRV WC3毒株与P7型VP4（93 - 96%）、G6型VP7（91 - 97%）、亚组（SG）I型VP6（96 - 99%）和NSP4基因群A（96 - 98%）的BRV毒株具有高度的氨基酸同一性。我们的结果证实并扩展了先前的研究，这些研究表明BRV WC3的VP4与P7原型BRV UK的VP4密切相关。此外，BRV WC3的VP6和VP7与SG I和G6 BRV NCDV及UK毒株的VP6和VP7非常相似。然而，BRV WC3的NSP4与P6原型BRV NCDV的NSP4比与BRV UK的NSP4关系更密切。
