• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

基于膜的反向转变循环：一种用于纯化植物源重组蛋白-弹性蛋白样多肽融合体的改进方法。

Membrane-based inverse transition cycling: an improved means for purifying plant-derived recombinant protein-elastin-like polypeptide fusions.

作者信息

Phan Hoang Trong, Conrad Udo

机构信息

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, Gatersleben 06466, Germany; E-Mail:

出版信息

Int J Mol Sci. 2011;12(5):2808-21. doi: 10.3390/ijms12052808. Epub 2011 Apr 29.

DOI:10.3390/ijms12052808
PMID:21686152
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3116158/
Abstract

Elastin-like peptide (ELP) was fused to two different avian flu H5N1 antigens and expressed in transgenic tobacco plants. The presence of the ELP tag enhanced the accumulation of the heterologous proteins in the tobacco leaves. An effective membrane-based Inverse Transition Cycling was developed to recover the ELPylated antigens and antibodies from plant material. The functionality of both the ELPylated neuraminidase and an ELPylated nanobody was demonstrated.

摘要

弹性蛋白样肽（ELP）与两种不同的禽流感H5N1抗原融合，并在转基因烟草植物中表达。ELP标签的存在增强了异源蛋白在烟草叶片中的积累。开发了一种基于膜的有效反向转变循环方法，用于从植物材料中回收ELP化的抗原和抗体。证明了ELP化神经氨酸酶和ELP化纳米抗体的功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/289e2b2b141f/ijms-12-02808f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/016d9a22e039/ijms-12-02808f1a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/f5e39b967b30/ijms-12-02808f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/8f712228ce4e/ijms-12-02808f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/e9b53cc489d8/ijms-12-02808f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/f6694d582f1a/ijms-12-02808f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/289e2b2b141f/ijms-12-02808f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/016d9a22e039/ijms-12-02808f1a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/f5e39b967b30/ijms-12-02808f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/8f712228ce4e/ijms-12-02808f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/e9b53cc489d8/ijms-12-02808f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/f6694d582f1a/ijms-12-02808f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fa5/3116158/289e2b2b141f/ijms-12-02808f6.jpg

相似文献

1
Membrane-based inverse transition cycling: an improved means for purifying plant-derived recombinant protein-elastin-like polypeptide fusions.基于膜的反向转变循环：一种用于纯化植物源重组蛋白-弹性蛋白样多肽融合体的改进方法。
Int J Mol Sci. 2011;12(5):2808-21. doi: 10.3390/ijms12052808. Epub 2011 Apr 29.
2
ELPylated haemagglutinins produced in tobacco plants induce potentially neutralizing antibodies against H5N1 viruses in mice.在烟草植物中产生的 ELPylated 血凝素可诱导小鼠产生针对 H5N1 病毒的潜在中和抗体。
Plant Biotechnol J. 2013 Jun;11(5):582-93. doi: 10.1111/pbi.12049. Epub 2013 Feb 11.
3
Influence of elastin-like polypeptide and hydrophobin on recombinant hemagglutinin accumulations in transgenic tobacco plants.弹性蛋白样多肽和疏水蛋白对转基因烟草植株中重组血凝素积累的影响。
PLoS One. 2014 Jun 10;9(6):e99347. doi: 10.1371/journal.pone.0099347. eCollection 2014.
4
Expression and immunogenicity of the mycobacterial Ag85B/ESAT-6 antigens produced in transgenic plants by elastin-like peptide fusion strategy.通过弹性蛋白样肽融合策略在转基因植物中产生的分枝杆菌Ag85B/ESAT-6抗原的表达及免疫原性
J Biomed Biotechnol. 2010;2010:274346. doi: 10.1155/2010/274346. Epub 2010 Apr 13.
5
Protein body formation in stable transgenic tobacco expressing elastin-like polypeptide and hydrophobin fusion proteins.稳定表达弹性蛋白样多肽和疏水蛋白融合蛋白的转基因烟草中的蛋白体形成。
BMC Biotechnol. 2013 May 10;13:40. doi: 10.1186/1472-6750-13-40.
6
Influence of elastin-like peptide fusions on the quantity and quality of a tobacco-derived human immunodeficiency virus-neutralizing antibody.弹性蛋白样肽融合物对烟草来源的人类免疫缺陷病毒中和抗体数量和质量的影响。
Plant Biotechnol J. 2009 Dec;7(9):899-913. doi: 10.1111/j.1467-7652.2009.00452.x. Epub 2009 Oct 13.
7
Elastin-like polypeptide fusions enhance the accumulation of recombinant proteins in tobacco leaves.类弹性蛋白多肽融合物可增强重组蛋白在烟草叶片中的积累。
Transgenic Res. 2007 Apr;16(2):239-49. doi: 10.1007/s11248-006-9026-2. Epub 2006 Nov 15.
8
Optimization of elastin-like polypeptide fusions for expression and purification of recombinant proteins in plants.用于在植物中表达和纯化重组蛋白的弹性蛋白样多肽融合体的优化
Biotechnol Bioeng. 2009 Jun 15;103(3):562-73. doi: 10.1002/bit.22278.
9
Expression in tobacco and purification of beak and feather disease virus capsid protein fused to elastin-like polypeptides.在烟草中表达并纯化融合了弹性蛋白样多肽的喙羽病病毒衣壳蛋白。
J Virol Methods. 2013 Jul;191(1):55-62. doi: 10.1016/j.jviromet.2013.03.028. Epub 2013 Apr 9.
10
Plant-based rapid production of recombinant subunit hemagglutinin vaccines targeting H1N1 and H5N1 influenza.基于植物的针对H1N1和H5N1流感的重组亚基血凝素疫苗的快速生产
Hum Vaccin. 2011 Jan-Feb;7 Suppl:41-50. doi: 10.4161/hv.7.0.14561. Epub 2011 Jan 1.

引用本文的文献

1
The HELP-UnaG Fusion Protein as a Bilirubin Biosensor: From Theory to Mature Technological Development.作为胆红素生物传感器的HELP-UnaG融合蛋白：从理论到成熟的技术发展
Molecules. 2025 Jan 21;30(3):439. doi: 10.3390/molecules30030439.
2
Membrane-based inverse-transition purification facilitates a rapid isolation of various spider-silk elastin-like polypeptide fusion proteins from extracts of transgenic tobacco.基于膜的逆转变性纯化法可从转基因烟草提取物中快速分离各种蜘蛛丝弹性蛋白样多肽融合蛋白。
Transgenic Res. 2024 Apr;33(1-2):21-33. doi: 10.1007/s11248-024-00375-z. Epub 2024 Apr 4.
3
Recent advances in expression and purification strategies for plant made vaccines.

本文引用的文献

1
Protein body-inducing fusions for high-level production and purification of recombinant proteins in plants.蛋白体诱导融合用于植物中重组蛋白的高水平生产和纯化。
Plant Biotechnol J. 2011 May;9(4):419-33. doi: 10.1111/j.1467-7652.2011.00596.x. Epub 2011 Feb 22.
2
Immunization with plant-expressed hemagglutinin protects chickens from lethal highly pathogenic avian influenza virus H5N1 challenge infection.植物表达的血凝素免疫可保护鸡免受致死性高致病性禽流感病毒 H5N1 攻击感染。
J Virol. 2010 Nov;84(22):12002-10. doi: 10.1128/JVI.00940-10. Epub 2010 Sep 1.
3
ELPylated anti-human TNF therapeutic single-domain antibodies for prevention of lethal septic shock.
植物源疫苗表达与纯化策略的最新进展。
Front Plant Sci. 2023 Nov 23;14:1273958. doi: 10.3389/fpls.2023.1273958. eCollection 2023.
4
Transient Expression of Dengue Virus NS1 Antigen in for Use as a Diagnostic Antigen.登革病毒NS1抗原的瞬时表达用于作为诊断抗原。
Front Plant Sci. 2020 Jan 16;10:1674. doi: 10.3389/fpls.2019.01674. eCollection 2019.
5
Transient expression of CCL21as recombinant protein in tomato.CCL21a重组蛋白在番茄中的瞬时表达。
Biotechnol Rep (Amst). 2017 Nov 26;17:10-15. doi: 10.1016/j.btre.2017.11.007. eCollection 2018 Mar.
6
Neutralizing immune responses induced by oligomeric H5N1-hemagglutinins from plants.植物源寡聚H5N1血凝素诱导的中和免疫反应
Vet Res. 2017 Sep 20;48(1):53. doi: 10.1186/s13567-017-0458-x.
7
Self-Assembling VHH-Elastin-Like Peptides for Photodynamic Nanomedicine.用于光动力纳米医学的自组装 VHH-弹性蛋白样肽。
Biomacromolecules. 2017 Apr 10;18(4):1302-1310. doi: 10.1021/acs.biomac.7b00064. Epub 2017 Mar 16.
8
Low-Tech, Pilot Scale Purification of a Recombinant Spider Silk Protein Analog from Tobacco Leaves.利用烟草叶片对重组蜘蛛丝蛋白类似物进行低技术含量的中试规模纯化。
Int J Mol Sci. 2016 Oct 9;17(10):1687. doi: 10.3390/ijms17101687.
9
In planta production of ELPylated spidroin-based proteins results in non-cytotoxic biopolymers.在植物中生产基于ELP化蜘蛛丝蛋白的蛋白质可产生无细胞毒性的生物聚合物。
BMC Biotechnol. 2015 Feb 19;15(1):9. doi: 10.1186/s12896-015-0123-2.
10
Synthesis and Immunogenicity Assessment of Elastin-Like Polypeptide-M2e Construct as an Influenza Antigen.作为流感抗原的弹性蛋白样多肽-M2e构建体的合成与免疫原性评估
Nano Life. 2014 Jun 1;4(2):1450004. doi: 10.1142/s1793984414500044.
ELPylated 抗人 TNF 治疗性单域抗体预防致死性脓毒性休克。
Plant Biotechnol J. 2011 Jan;9(1):22-31. doi: 10.1111/j.1467-7652.2010.00523.x.
4
Expression and immunogenicity of the mycobacterial Ag85B/ESAT-6 antigens produced in transgenic plants by elastin-like peptide fusion strategy.通过弹性蛋白样肽融合策略在转基因植物中产生的分枝杆菌Ag85B/ESAT-6抗原的表达及免疫原性
J Biomed Biotechnol. 2010;2010:274346. doi: 10.1155/2010/274346. Epub 2010 Apr 13.
5
Purification of plant-derived antibodies through direct immobilization of affinity ligands on cellulose.通过将亲和配体直接固定在纤维素上，从植物中纯化抗体。
J Agric Food Chem. 2010 Mar 24;58(6):3451-9. doi: 10.1021/jf9040657.
6
Elastin-like polypeptides revolutionize recombinant protein expression and their biomedical application.弹性蛋白样多肽彻底改变了重组蛋白的表达及其在生物医学中的应用。
Trends Biotechnol. 2010 Jan;28(1):37-45. doi: 10.1016/j.tibtech.2009.10.004. Epub 2009 Nov 10.
7
Influence of elastin-like peptide fusions on the quantity and quality of a tobacco-derived human immunodeficiency virus-neutralizing antibody.弹性蛋白样肽融合物对烟草来源的人类免疫缺陷病毒中和抗体数量和质量的影响。
Plant Biotechnol J. 2009 Dec;7(9):899-913. doi: 10.1111/j.1467-7652.2009.00452.x. Epub 2009 Oct 13.
8
A plant-produced influenza subunit vaccine protects ferrets against virus challenge.一种植物生产的流感亚单位疫苗可保护雪貂免受病毒攻击。
Influenza Other Respir Viruses. 2008 Jan;2(1):33-40. doi: 10.1111/j.1750-2659.2008.00037.x.
9
Optimization of elastin-like polypeptide fusions for expression and purification of recombinant proteins in plants.用于在植物中表达和纯化重组蛋白的弹性蛋白样多肽融合体的优化
Biotechnol Bioeng. 2009 Jun 15;103(3):562-73. doi: 10.1002/bit.22278.
10
Immunogenicity of hemagglutinin from A/Bar-headed Goose/Qinghai/1A/05 and A/Anhui/1/05 strains of H5N1 influenza viruses produced in Nicotiana benthamiana plants.在本氏烟草植株中产生的H5N1流感病毒A/斑头雁/青海/1A/05株和A/安徽/1/05株血凝素的免疫原性。
Vaccine. 2009 May 26;27(25-26):3467-70. doi: 10.1016/j.vaccine.2009.01.051. Epub 2009 Feb 5.