Warren Alessandra, Cogger Victoria C, Fraser Robin, Deleve Laurie D, McCuskey Robert S, Le Couteur David G
Centre for Education and Research on Ageing, Concord Hospital and the ANZAC Research Institute, The University of Sydney, Sydney NSW 2139, Australia.
Curr Gerontol Geriatr Res. 2011;2011:439835. doi: 10.1155/2011/439835. Epub 2011 May 18.
Aging is associated with marked changes in the hepatic sinusoid, yet the effect of old age on hepatic stellate cells (HSC) has not been well described. Transmission electron microscopy and immunohistochemistry were used to study the effects of aging on HSC in livers from rats (3-4 mths versus 24-27 mths) and mice (2-3 mths versus 20-22 mths). Desmin-positive HSC doubled in old age in both mice and rats. Alpha-smooth muscle actin- (αSMA-) positive cells did not increase significantly and remained only a small percentage of desmin-positive cells. Electron microscopy revealed that old age is associated with HSC that have a substantial increase in the number of lipid droplets which are larger in diameter. There was also a marked increase of HSC that protruded into the sinusoidal lumen in old mice. In conclusion, old age is associated with hyperplasia of HSC that are not activated and are engorged with lipid droplets.
衰老与肝血窦的显著变化相关，但衰老对肝星状细胞（HSC）的影响尚未得到充分描述。采用透射电子显微镜和免疫组织化学方法研究衰老对大鼠（3 - 4个月与24 - 27个月）和小鼠（2 - 3个月与20 - 22个月）肝脏中HSC的影响。结蛋白阳性的HSC在老年小鼠和大鼠中均增加了一倍。α - 平滑肌肌动蛋白（αSMA）阳性细胞没有显著增加，仅占结蛋白阳性细胞的一小部分。电子显微镜显示，衰老与HSC中脂滴数量大幅增加且直径更大有关。老年小鼠中突入血窦腔的HSC也显著增加。总之，衰老与未被激活且充满脂滴的HSC增生有关。
