Caviedes-Bucheli J, Rios-Osorio N, Rey-Rojas M, Laguna-Rivero F, Azuero-Holguin M M, Diaz L E, Curtidor H, Castaneda-Ramirez J J, Munoz H R
Centro de Investigaciones Odontologicas, Pontificia Universidad Javeriana, Bogota, Colombia.
Postgraduate Endodontics Department, Institucion Universitaria Colegios de Colombia, Bogota, Colombia.
Int Endod J. 2018 Dec;51(12):1358-1366. doi: 10.1111/iej.12952. Epub 2018 Jun 7.
To quantify Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament from premolars after root canal preparation with Reciproc Blue, WaveOne Gold, XP EndoShaper and hand files.
A total of 50 human periodontal ligament samples were obtained from healthy mandibular premolars where extraction was indicated for orthodontic reasons. Prior to extraction, 40 of these premolars were equally divided into four groups, and root canals were prepared using four different systems: Reciproc Blue, WaveOne Gold, XP EndoShaper and a hand instrumentation technique. The remaining 10 healthy premolars were extracted without treatment and served as a negative control group. All periodontal ligament samples were processed, and SP and CGRP were measured by radioimmunoassay. The Kruskal-Wallis test was used to establish significant differences between groups and LSD post hoc comparisons were also performed.
Greater SP and CGRP values were found in the hand instrumentation group, followed by the XP EndoShaper, WaveOne Gold and the Reciproc groups. The lower SP and CGRP values were for the healthy periodontal ligament group. The Kruskal-Wallis test revealed significant differences between groups (P < 0.05). Post hoc Least Significant Difference (LSD) tests revealed significant differences (P < 0.05) in SP and CGRP expression between all the comparisons except for the Reciproc Blue and WaveOne Gold group (P > 0.05).
All the root canal preparation techniques tested increased SP and CGRP expression in human periodontal ligament, with hand files and XP EndoShaper instruments being associated with greater neuropeptide release compared to Reciproc Blue and WaveOne Gold files.
量化使用Reciproc Blue、WaveOne Gold、XP EndoShaper和手动锉进行根管预备后，健康人前磨牙牙周膜中P物质（SP）和降钙素基因相关肽（CGRP）的表达。
从因正畸需要拔除的健康下颌前磨牙中获取50份人牙周膜样本。在拔除前，将其中40颗前磨牙平均分为四组，使用四种不同的系统进行根管预备：Reciproc Blue、WaveOne Gold、XP EndoShaper和一种手动器械技术。其余10颗健康前磨牙未经处理即拔除，作为阴性对照组。对所有牙周膜样本进行处理，并通过放射免疫测定法测量SP和CGRP。采用Kruskal-Wallis检验确定组间的显著差异，并进行LSD事后比较。
手动器械组中SP和CGRP的值更高，其次是XP EndoShaper组、WaveOne Gold组和Reciproc组。健康牙周膜组的SP和CGRP值较低。Kruskal-Wallis检验显示组间存在显著差异（P < 0.05）。事后最小显著差异（LSD）检验显示，除Reciproc Blue组和WaveOne Gold组之间（P > 0.05）外，所有比较中的SP和CGRP表达均存在显著差异（P < 0.05）。
所有测试的根管预备技术均增加了人牙周膜中SP和CGRP的表达，与Reciproc Blue和WaveOne Gold锉相比，手动锉和XP EndoShaper器械与更多的神经肽释放相关。
