School of Dentistry, Universidad Santo Tomas, Floridablanca Bucaramanga, Santander, Colombia.
Int Endod J. 2013 May;46(5):419-26. doi: 10.1111/iej.12005. Epub 2012 Oct 12.
To quantify the effect of two single-file reciprocating root canal preparation systems on Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament (PDL).
Forty PDL samples were obtained from healthy premolars where extraction was indicated for orthodontic reasons. Prior to extraction, 20 of these premolars were divided equally in two groups, and then, root canals were prepared using one of two different single-file systems: WaveOne and Reciproc. Ten premolars were prepared with hand files and served as a positive control group. The remaining 10 premolars where extracted without treatment and served as a negative control group. All PDL samples were processed, and SP and CGRP were measured by radioimmunoassay.
Greater SP and CGRP expression were found in the hand instrumentation group (1.220 pmol SP and 0.084 pmol CGRP per mg of PDL), followed by the WaveOne group (0.908 pmol SP and 0.046 pmol CGRP per mg of PDL) and the Reciproc group (0.511 pmol SP and 0.022 pmol CGRP per mg of PDL). The lower SP and CGRP values were associated with the intact control group (0.453 pmol SP and 0.018 pmol CGRP per mg of PDL). The Kruskal-Wallis test revealed significant differences between groups (P < 0.001). Post hoc Tukey HSD tests revealed significant differences in SP and CGRP expression between intact teeth in the control group and all the other groups (P < 0.001) except with the Reciproc group (P = 0.165 and P = 0.42 for SP and CGRP, respectively). Hand instrumentation was associated with significant differences with all the other groups (P < 0.001). Differences between the WaveOne and Reciproc groups were also significant (P < 0.001).
Substance P and CGRP expression in PDL cells increased when teeth were prepared with WaveOne as well as with hand instrumentation. Reciproc maintained SP and CGRP levels in line with the negative control group.
定量研究两种单文件根管预备系统对健康人牙周韧带（PDL）中 P 物质（SP）和降钙素基因相关肽（CGRP）表达的影响。
从因正畸需要拔除的健康前磨牙中获得 40 个 PDL 样本。在拔牙前，将其中 20 个前磨牙等分为两组，然后使用两种不同的单文件系统之一进行根管预备：WaveOne 和 Reciproc。10 个前磨牙用手用器械预备作为阳性对照组。其余 10 个前磨牙未经处理作为阴性对照组。对所有 PDL 样本进行处理，并用放射免疫法测量 SP 和 CGRP。
手用器械组（PDL 中每毫克 SP 为 1.220 pmol，CGRP 为 0.084 pmol）、WaveOne 组（PDL 中每毫克 SP 为 0.908 pmol，CGRP 为 0.046 pmol）和 Reciproc 组（PDL 中每毫克 SP 为 0.511 pmol，CGRP 为 0.022 pmol）的 SP 和 CGRP 表达更高。与完整对照组（PDL 中每毫克 SP 为 0.453 pmol，CGRP 为 0.018 pmol）相比，SP 和 CGRP 值较低。Kruskal-Wallis 检验显示组间差异有统计学意义（P < 0.001）。事后 Tukey HSD 检验显示，完整对照组（P < 0.001）、手用器械组（P < 0.001）、WaveOne 组（P < 0.001）和 Reciproc 组（P < 0.001）之间的 SP 和 CGRP 表达差异均有统计学意义，但 Reciproc 组与 Reciproc 组之间无统计学差异（P = 0.165 和 P = 0.42）。手用器械与其他组之间差异有统计学意义（P < 0.001）。WaveOne 组和 Reciproc 组之间的差异也有统计学意义（P < 0.001）。
WaveOne 以及手用器械预备牙时，PDL 细胞中的 P 物质和降钙素基因相关肽表达增加。Reciproc 维持 SP 和 CGRP 水平与阴性对照组一致。
