Center for Molecular and Vascular Biology, KU Leuven, Leuven, Belgium.
Thromb Res. 2012 Jan;129(1):74-9. doi: 10.1016/j.thromres.2011.05.023.
To evaluate associations between adiposity and coagulation or inflammation profile, obese wild-type C57Bl/6 mice were subjected to drastic caloric restriction by switching from a high fat diet to restricted normal chow. After 6 weeks, total body weights as well as subcutaneous and gonadal adipose tissue mass were markedly reduced, associated with adipocyte hypotrophy (all p<0.001). Weight reduction was associated with markedly reduced plasma levels of plasminogen activator inhibitor-1, Factor VII and Factor VIII. Reduced oxidative stress and inflammation following weight reduction is supported by significantly lower expression in adipose tissues of pro-inflammatory interleukin-6, higher expression of anti-oxidant catalase, superoxide dismutase 1 and glutathione peroxidase 1, and lower plasma levels of C-reactive protein. Furthermore, reduced levels of leptin and enhanced levels of adiponectin were observed, whereas cholesterol and triglyceride levels were reduced. The content of structurally intact collagen fibers was significantly higher in subcutaeneous and gonadal adipose tissues after caloric restriction. Thus, caloric restriction and drastic weight loss in obese mice is associated with improved plasma coagulation profile and with reduced oxidative stress and inflammation in the adipose tissues.
为了评估肥胖与凝血或炎症特征之间的关系，将肥胖野生型 C57Bl/6 小鼠从高脂肪饮食切换到限制正常食物，进行严格的热量限制。6 周后，体重以及皮下和性腺脂肪组织质量明显减少，与脂肪细胞萎缩有关（均 p<0.001）。体重减轻与纤溶酶原激活物抑制剂-1、因子 VII 和因子 VIII 的血浆水平显著降低有关。体重减轻后氧化应激和炎症减少得到支持，脂肪组织中促炎白细胞介素 6 的表达降低，抗氧化剂过氧化氢酶、超氧化物歧化酶 1 和谷胱甘肽过氧化物酶 1 的表达增加，血浆 C-反应蛋白水平降低。此外，观察到瘦素水平降低和脂联素水平升高，而胆固醇和甘油三酯水平降低。热量限制后，皮下和性腺脂肪组织中结构完整的胶原纤维含量明显升高。因此，肥胖小鼠的热量限制和体重急剧下降与改善的血浆凝血特征以及脂肪组织中减少的氧化应激和炎症有关。
