Egashira Nobuaki, Nogami Ai, Iwasaki Katsunori, Ishibashi Ayumi, Uchida Naoki, Takasaki Kotaro, Mishima Kenichi, Nishimura Ryoji, Oishi Ryozo, Fujiwara Michihiro
Department of Neuropharmacology, Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.
J Pharmacol Sci. 2011;116(3):316-20. Epub 2011 Jun 18.
In the present study, we investigated the effect of the Kampo medicine Yokukansan (YKS) on pentobarbital-induced sleep in group-housed and socially isolated mice. Socially isolated mice showed shorter sleeping time than the group-housed mice. YKS (300 mg/kg, p.o.) prolonged the pentobarbital-induced sleeping time in socially isolated mice without affecting pentobarbital sleep in group-housed mice. The prolongation of sleeping time by YKS was reversed by bicuculline (3 mg/kg, i.p.) and flumazenil (3 mg/kg, i.p.), but not WAY100635. These findings suggest that the GABA(A) - benzodiazepine receptor complex, but not 5-HT(1A) receptors, is involved in the reversal effect of YKS on the decrease of pentobarbital sleep by social isolation.
在本研究中，我们调查了汉方药“抑肝散”（YKS）对群居和社会隔离小鼠戊巴比妥诱导睡眠的影响。社会隔离小鼠的睡眠时间比群居小鼠短。YKS（300mg/kg，口服）延长了社会隔离小鼠中戊巴比妥诱导的睡眠时间，而不影响群居小鼠的戊巴比妥睡眠。YKS延长睡眠时间的作用被荷包牡丹碱（3mg/kg，腹腔注射）和氟马西尼（3mg/kg，腹腔注射）逆转，但未被WAY100635逆转。这些发现表明，γ-氨基丁酸A（GABA(A)）-苯二氮䓬受体复合物而非5-羟色胺1A（5-HT(1A)）受体参与了YKS对社会隔离导致的戊巴比妥睡眠减少的逆转作用。
