Liu Jiaolian, Xia Xiaobo, Xiong Siqi, Le Yunzheng, Xu Huizhuo
Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha 410008, China.
Eye Sci. 2011 Jun;26(2):85-90, 120. doi: 10.3969/j.issn.1000-4432.2011.02.017.
To investigate the significance of netrin-1 and vascular endothelial growth factor (VEGF) in the pathogenesis of retinal angiogenesis, the levels of netrin-1 and VEGF in the vitreous fluid and serum of the proliferative diabetic retinopathy (PDR) and non-proliferative diabetic retinopathy (non-PDR) patients were measured. We then determined the netrin-1 and VEGF expression in the oxygen induced retinopathy (OIR) mice retinas.
A total of 18 eyes from 18 patients were included in our study and 10 of them were collected from PDR patients and 8 from non-PDR patients. Undiluted vitreous fluid samples were collected during pars plana vitrectomy. Appropriate blood samples were collected if possible. Netrin-1 and VEGF levels in the vitreous fluid and plasma were determined by Enzyme-linked Immunosorbent Assays. OIR mice models were established, and netrin-1 and VEGF levels were determined by immunohistochemistry analysis.
The levels of netrin-1 and VEGF in the vitreous of PDR patients were significantly higher than those in the controls (Mediannetrin-1=509.94 vs. 85.91 pg/ml, P<0.001 and Median VEGF=762.60 vs. 77.52 pg pg/ml, P<0.001). Netrin-1 was mainly expressed in GCL and INL of the retina in mice. Both netrin-1 and VEGF were up-regulated in OIR mice.
Netrin-1 and VEGF levels were elevated in vitreous fluid of the PDR patients and the OIR mice retinas. Therefore, netrin-1 may play important roles in pathological retinal angiogenesis.
为研究netrin-1和血管内皮生长因子（VEGF）在视网膜血管生成发病机制中的意义，我们检测了增殖性糖尿病视网膜病变（PDR）和非增殖性糖尿病视网膜病变（non-PDR）患者玻璃体液和血清中netrin-1和VEGF的水平。然后我们测定了氧诱导视网膜病变（OIR）小鼠视网膜中netrin-1和VEGF的表达。
本研究共纳入18例患者的18只眼，其中10只来自PDR患者，8只来自non-PDR患者。在玻璃体切割术中收集未稀释的玻璃体液样本。如有可能，采集适量血液样本。采用酶联免疫吸附测定法测定玻璃体液和血浆中netrin-1和VEGF的水平。建立OIR小鼠模型，通过免疫组织化学分析测定netrin-1和VEGF水平。
PDR患者玻璃体内netrin-1和VEGF水平显著高于对照组（netrin-1中位数=509.94 vs. 85.91 pg/ml，P<0.001；VEGF中位数=762.60 vs. 77.52 pg/ml，P<0.001）。Netrin-1主要在小鼠视网膜的神经节细胞层（GCL）和内核层（INL）表达。在OIR小鼠中，netrin-1和VEGF均上调。
PDR患者玻璃体液和OIR小鼠视网膜中netrin-1和VEGF水平升高。因此，netrin-1可能在病理性视网膜血管生成中起重要作用。
