睡茄根、茎和叶对多种人类癌细胞系的体外抗癌活性
In Vitro Anticancer Activity of the Root, Stem and Leaves of Withania Somnifera against Various Human Cancer Cell Lines.
作者信息
Yadav B, Bajaj A, Saxena M, Saxena A K
机构信息
Advanced Materials and Processes Research Institute (CSIR), Bhopal-462 026, India.
出版信息
Indian J Pharm Sci. 2010 Sep;72(5):659-63. doi: 10.4103/0250-474X.78543.
Abstract
Withania Somnifera Dunal know as Ashwagandha belong Solanaceae family. It is extensively used in most of the Indian herbal pharmaceuticals and nutraceuticals. The current study, evaluate in vitro cytotoxicity in 50% ethanol extract of root, stem and leaves of Withania Somnifera against five human cancer cell lines of four different tissues i.e. PC-3, DU-145 (prostrate), HCT-15 (colon), A-549 (lung) and IMR-32 (neuroblastoma). Root, stem and leaves extracts showed cytotoxicity activity ranging 0-98% depending on the cell lines but maximum activity was found in 50% ethanol extract of leaves of Withania Somnifera. Ethanol extract of leaves obtained from treatments T2, T3, T4 and T5 showed strong activity against PC-3 and HCT-15 with 80-98% growth inhibition, while the 50% ethanol extract of leaves from T1 treatment showed a minimum of 39% and T3 treatment showed a maximum of 98% growth inhibition against HCT-15. This investigation is the first report of the anticancer activity in various parts of Withania Somnifera cultivated in fly ash amended soil.
摘要
睡茄（Withania Somnifera Dunal），又名南非醉茄，属于茄科。它在大多数印度草药药品和营养保健品中被广泛使用。本研究评估了睡茄根、茎和叶的50%乙醇提取物对四种不同组织的五种人类癌细胞系，即PC-3、DU-145（前列腺）、HCT-15（结肠）、A-549（肺）和IMR-32（神经母细胞瘤）的体外细胞毒性。根、茎和叶提取物的细胞毒性活性在0-98%之间，具体取决于细胞系，但睡茄叶的50%乙醇提取物中发现的活性最高。从处理T2、T3、T4和T5获得的叶乙醇提取物对PC-3和HCT-15表现出强烈活性，生长抑制率为80-98%，而T1处理的叶50%乙醇提取物对HCT-15的生长抑制率最低为39%，T3处理的最高为98%。本研究是关于在粉煤灰改良土壤中种植的睡茄各部分抗癌活性的首次报道。
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