Laboratory of Physiology and Ecology, Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Japan.
Fish Physiol Biochem. 2012 Apr;38(2):483-91. doi: 10.1007/s10695-011-9528-6. Epub 2011 Jun 22.
Effects of etidronate on the calcification of scales and ribs were investigated in goldfish. Daily intraperitoneal injections of etidronate at doses of 1 and 10 mgP/kg body weight for 2 weeks inhibited calcification of ontogenic scales and ribs without affecting the accretion of organic matrices. Removal of some scales induced their regeneration within the two-week period. Their newly formed organic matrix was fully uncalcified in fish treated with 10 mgP/kg, whereas in those treated with 1 mgP/kg, the regenerating scales were only partially calcified. Daily administration of etidronate 10 mgP/kg resulted in an increase of body weight. These results suggested that the inhibitory effect of etidronate on the calcification of osseous tissues in mammals can be expected also on comparable tissues in fishes. An appropriate dose of etidronate that inhibits hard tissue calcification but not affects the body growth in fish seemed to exist between 1 and 10 mgP/kg.
我们研究了依替膦酸盐对金鱼鳞片和肋骨钙化的影响。连续 2 周每天经腹腔注射依替膦酸盐 1 和 10mgP/kg 体重，剂量可抑制个体发育中的鳞片和肋骨的钙化，而不影响有机基质的形成。在此期间，去除一些鳞片可诱导其再生。在 10mgP/kg 剂量处理的鱼中，新形成的有机基质完全未钙化，而在 1mgP/kg 剂量处理的鱼中，再生鳞片仅部分钙化。每天给予 10mgP/kg 的依替膦酸盐可导致体重增加。这些结果表明，依替膦酸盐对哺乳动物骨组织钙化的抑制作用也可预期在鱼类的类似组织中发生。在 1 至 10mgP/kg 之间，似乎存在一个抑制硬组织钙化但不影响鱼类生长的依替膦酸盐适宜剂量。
