Oral Biology Department, Faculty of Oral and Dental Medicine, Cairo University, 51 El Hadara st., Nerco, Degla, Cairo, Egypt.
Arch Oral Biol. 2011 Nov;56(11):1339-46. doi: 10.1016/j.archoralbio.2011.05.006. Epub 2011 Jun 21.
The aim of the present study was to compare between two antioxidant treatments in prevention of fenitrothion induced toxicity on rat parotid salivary gland.
Forty adult male Wistar rats with an average weight of 120-150 g were randomised into 4 groups, control (group I), fenitrothion administration (group II), fenitrothion administration 1h after green tea extract or Nigella sativa oil extract administration (groups III and IV respectively). The rats were then sacrificed after 28 days. The submandibular salivary glands were examined histologically, immunohistochemically and ultrastructurally.
Histopathologically the fenitrothion group showed sign of acini degeneration represented by loss of normal architecture (amalgamation). The nuclei of the acinar cells revealed different sizes and shape (polymorphism). The acini relatively preserved their shape in both prophylactic groups (III and IV). Histomorphometric analysis showed significant increase in the optical density of caspase-3 cleaved activity in all experimental groups (p=0.0001). A significant difference was observed between both prophylactic experimental groups III and IV (p=0.0039). Ultrastructurally, the nuclei of serous acini in group II appeared pyknotic with segregation of chromatin. Condensation of the chromatin at the periphery of the nucleus was observed in the nuclei of group III, Clumping of the chromatin with darkly stained pyknotic nucleus was detected in group IV.
In a rat model the administration of natural antioxidants could be of beneficial effect on prevention of cytotoxicity induced by organophosphorous compounds. However, green tea showed more promising results than that of Nigella sativa.
本研究旨在比较两种抗氧化剂治疗方案在预防对硫磷诱导的大鼠腮腺毒性中的作用。
40 只成年雄性 Wistar 大鼠，平均体重 120-150g，随机分为 4 组，对照组（I 组）、对硫磷给药组（II 组）、对硫磷给药 1 小时后给予绿茶提取物或黑种草油提取物组（III 组和 IV 组）。28 天后处死大鼠。对颌下腺进行组织学、免疫组织化学和超微结构检查。
组织病理学上，对硫磷组表现出腺泡退化的迹象，表现为正常结构丧失（融合）。腺泡细胞的核显示出不同的大小和形状（多形性）。在两种预防性给药组（III 组和 IV 组）中，腺泡相对保持其形状。组织形态计量分析显示，所有实验组的 caspase-3 活性裂解产物的光密度均显著增加（p=0.0001）。两组预防性实验组（III 组和 IV 组）之间存在显著差异（p=0.0039）。超微结构上，II 组的浆液性腺泡核呈固缩，染色质分离。III 组的核观察到染色质在核的周边浓缩，IV 组的核观察到染色质聚集，核呈深染固缩。
在大鼠模型中，天然抗氧化剂的给药可能对预防有机磷化合物诱导的细胞毒性具有有益作用。然而，绿茶的效果比黑种草油更有前景。
