制备抗 CD40 抗体修饰的磁性 PCL-PEG-PCL 微球。

Preparation of anti-CD40 antibody modified magnetic PCL-PEG-PCL microspheres.

机构信息

State Key Laboratory of Biotherapy and Cancer Center West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, P R. China.

出版信息

J Biomed Nanotechnol. 2011 Apr;7(2):285-91. doi: 10.1166/jbn.2011.1280.

DOI:10.1166/jbn.2011.1280

PMID:21702366

Abstract

Antibody modified magnetic polymeric microspheres have potential biomedical application. In this paper, anti-CD40 antibody modified magnetic poly(epsilon-caprolactone)-poly(ethylene glycol)-poly(epsilon-caprolactone) (PCL-PEG-PCL, PCEC) microspheres were prepared. First, PCL-PEG-PCL triblock copolymer was synthesized by ring-opening polymerization, followed by reaction with succinic anhydride, creating carboxylated PCL-PEG-PCL copolymer. Then, magnetite nanoparticles were encapsulated into carboxylated PCL-PEG-PCL microspheres, forming magnetic PCL-PEG-PCL microspheres with carboxyl group on their surface. Catalyzed by EDC/NHS, the anti-CD40 antibody was linked to these magnetic PCL-PEG-PCL microspheres, thus forming anti-CD40 modified PCL-PEG-PCL microspheres. These anti-CD40 antibody modified magnetic PCL-PEG-PCL microspheres may have potential application in cell separation.

摘要

抗体修饰的磁性高分子微球具有潜在的生物医学应用。本文制备了抗 CD40 抗体修饰的磁性聚（ε-己内酯）-聚（乙二醇）-聚（ε-己内酯）（PCL-PEG-PCL，PCEC）微球。首先，通过开环聚合合成 PCL-PEG-PCL 三嵌段共聚物，然后与琥珀酸酐反应，制得羧基化 PCL-PEG-PCL 共聚物。然后，将磁性纳米粒子包封到羧基化 PCL-PEG-PCL 微球中，在其表面形成带有羧基的磁性 PCL-PEG-PCL 微球。在 EDC/NHS 的催化下，将抗 CD40 抗体连接到这些磁性 PCL-PEG-PCL 微球上，从而形成抗 CD40 修饰的 PCL-PEG-PCL 微球。这些抗 CD40 抗体修饰的磁性 PCL-PEG-PCL 微球可能在细胞分离中有潜在的应用。