焦磷酸酶对大肠杆菌的生长至关重要。
Pyrophosphatase is essential for growth of Escherichia coli.
作者信息
Chen J, Brevet A, Fromant M, Lévêque F, Schmitter J M, Blanquet S, Plateau P
机构信息
Laboratoire de Biochimie, 240 du Centre National de la Recherche Scientifique, Ecole Polytechnique, Palaiseau, France.
出版信息
J Bacteriol. 1990 Oct;172(10):5686-9. doi: 10.1128/jb.172.10.5686-5689.1990.
Abstract
The ppa gene for inorganic pyrophosphatase is essential for the growth of Escherichia coli. A recombinant with a chromosomal ppa::Kanr lesion and a temperature-sensitive replicon with a ppa+ gene showed a temperature-sensitive growth phenotype, and a mutant with the sole ppa+ gene under control of the lac promoter showed inducer-dependent growth. When the lacp-ppa mutant was subcultured without inducer, the pyrophosphatase level decreased, the PPi level increased, and growth stopped. Cellular PPi reached 16 mM about 6 h after growth arrest without loss of cell viability.
摘要
无机焦磷酸酶的ppa基因对大肠杆菌的生长至关重要。一个带有染色体ppa::Kanr损伤和携带ppa+基因的温度敏感型复制子的重组体表现出温度敏感型生长表型，并且一个在lac启动子控制下仅含有ppa+基因的突变体表现出诱导物依赖性生长。当lacp-ppa突变体在没有诱导物的情况下传代培养时，焦磷酸酶水平降低，焦磷酸（PPi）水平升高，生长停止。在生长停止后约6小时，细胞内PPi达到16 mM，而细胞活力并未丧失。
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