在大肠杆菌K-12细胞中，细胞内焦磷酸（PPi）浓度并不直接依赖于无机焦磷酸酶的含量。
Intracellular PPi concentration is not directly dependent on amount of inorganic pyrophosphatase in Escherichia coli K-12 cells.
作者信息
Kukko-Kalske E, Lintunen M, Inen M K, Lahti R, Heinonen J
机构信息
Department of Biochemistry, University of Turku, Finland.
出版信息
J Bacteriol. 1989 Aug;171(8):4498-500. doi: 10.1128/jb.171.8.4498-4500.1989.
Abstract
No correlation was observed between the level of inorganic pyrophosphatase (PPase) and the intracellular concentration of PPi in Escherichia coli cells. In exponentially growing cells the intracellular PPi concentration was in every case 1.5 nmol/mg (dry weight) or about 0.5 mM, even though the amount of PPase was varied from 15 to 2,600% of the control amount by mutation or by using a multicopy plasmid with an inserted gene (ppa) encoding PPase. The PPi concentration could, however, be increased or decreased from the control level under some stressful conditions.
摘要
在大肠杆菌细胞中，未观察到无机焦磷酸酶（PPase）水平与细胞内焦磷酸（PPi）浓度之间存在相关性。在指数生长期的细胞中，无论通过突变或使用带有插入编码PPase基因（ppa）的多拷贝质粒，使PPase的量在对照量的15%至2600%之间变化，细胞内PPi浓度在每种情况下均为1.5 nmol/mg（干重）或约0.5 mM。然而，在某些应激条件下，PPi浓度可能会从对照水平升高或降低。
相似文献
1
Intracellular PPi concentration is not directly dependent on amount of inorganic pyrophosphatase in Escherichia coli K-12 cells.
J Bacteriol. 1989 Aug;171(8):4498-500. doi: 10.1128/jb.171.8.4498-4500.1989.
2
Diphosphate concentration does not correlate with the level of inorganic diphosphatase in Escherichia coli.
Folia Microbiol (Praha). 1984;29(4):282-7. doi: 10.1007/BF02875958.
3
Catalysis by Escherichia coli inorganic pyrophosphatase: pH and Mg2+ dependence.
Biochemistry. 1996 Apr 16;35(15):4655-61. doi: 10.1021/bi952635u.
4
A hybrid mutant form of Escherichia coli inorganic pyrophosphatase.
Biochemistry (Mosc). 1997 Mar;62(3):233-6.
5
Inorganic pyrophosphate and inorganic pyrophosphatase in Escherichia coli.
Int J Biochem. 1985;17(5):575-80. doi: 10.1016/0020-711x(85)90288-5.
6
Vacuolar H-Pyrophosphatase and Cytosolic Soluble Pyrophosphatases Cooperatively Regulate Pyrophosphate Levels in .
Plant Cell. 2018 May;30(5):1040-1061. doi: 10.1105/tpc.17.00911. Epub 2018 Apr 24.
7
Mechanism of Ca2+-induced inhibition of Escherichia coli inorganic pyrophosphatase.
Biochemistry (Mosc). 2000 Mar;65(3):373-87.
8
Pyrophosphatase is essential for growth of Escherichia coli.
J Bacteriol. 1990 Oct;172(10):5686-9. doi: 10.1128/jb.172.10.5686-5689.1990.
9
Characterization of the 5' flanking region of the Escherichia coli ppa gene encoding inorganic pyrophosphatase: mutations in the ribosome-binding site decrease the level of ppa mRNA.
J Gen Microbiol. 1991 Nov;137(11):2517-23. doi: 10.1099/00221287-137-11-2517.
10
Effectory site in Escherichia coli inorganic pyrophosphatase is revealed upon mutation at the intertrimeric interface.
IUBMB Life. 2003 Jan;55(1):37-41. doi: 10.1080/1521654031000072139.
引用本文的文献
1
An empirical model of aminoacylation kinetics for E. coli class I and II aminoacyl tRNA synthetases.
PLoS Comput Biol. 2025 Aug 12;21(8):e1013353. doi: 10.1371/journal.pcbi.1013353. eCollection 2025 Aug.
2
Step-by-Step Regulation of Productive and Abortive Transcription Initiation by Pyrophosphorolysis.
J Mol Biol. 2022 Jul 15;434(13):167621. doi: 10.1016/j.jmb.2022.167621. Epub 2022 May 6.
3
Intermediary conformations linked to the directionality of the aminoacylation pathway of nonribosomal peptide synthetases.
RSC Chem Biol. 2021 Mar 4;2(3):843-854. doi: 10.1039/d0cb00220h. eCollection 2021 Jun 1.
4
Trade-Offs between Speed, Accuracy, and Dissipation in tRNA Aminoacylation.
J Phys Chem Lett. 2020 May 21;11(10):4001-4007. doi: 10.1021/acs.jpclett.0c01073. Epub 2020 May 6.
5
The Role of Pyrophosphorolysis in the Initiation-to-Elongation Transition by E. coli RNA Polymerase.
J Mol Biol. 2019 Jun 28;431(14):2528-2542. doi: 10.1016/j.jmb.2019.04.020. Epub 2019 Apr 26.
6
Pyrophosphate Stimulates the Phosphate-Sodium Symporter of Acidocalcisomes and Vacuoles.
mSphere. 2019 Apr 3;4(2):e00045-19. doi: 10.1128/mSphere.00045-19.
7
Identification of Phosphorylation Sites Altering Pollen Soluble Inorganic Pyrophosphatase Activity.
Plant Physiol. 2017 Mar;173(3):1606-1616. doi: 10.1104/pp.16.01450. Epub 2017 Jan 26.
8
Experimental and Metabolic Modeling Evidence for a Folate-Cleaving Side-Activity of Ketopantoate Hydroxymethyltransferase (PanB).
Front Microbiol. 2016 Mar 31;7:431. doi: 10.3389/fmicb.2016.00431. eCollection 2016.
9
When Too Much ATP Is Bad for Protein Synthesis.
J Mol Biol. 2015 Aug 14;427(16):2586-2594. doi: 10.1016/j.jmb.2015.06.021. Epub 2015 Jul 4.
10
Inorganic pyrophosphatase defects lead to cell cycle arrest and autophagic cell death through NAD+ depletion in fermenting yeast.
J Biol Chem. 2013 May 3;288(18):13082-92. doi: 10.1074/jbc.M112.439349. Epub 2013 Mar 11.
本文引用的文献
1
Metabolic alterations mediated by 2-ketobutyrate in Escherichia coli K12.
Mol Gen Genet. 1984;193(3):473-8. doi: 10.1007/BF00382086.
2
Low-copy-number plasmid-cloning vectors amplifiable by derepression of an inserted foreign promoter.
Gene. 1984 Apr;28(1):45-54. doi: 10.1016/0378-1119(84)90086-6.
3
Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.
Gene. 1983 Dec;26(1):101-6. doi: 10.1016/0378-1119(83)90040-9.
4
The intracellular concentration of pyrophosphate in the batch culture of Escherichia coli.
Eur J Biochem. 1982 Oct;127(2):347-9. doi: 10.1111/j.1432-1033.1982.tb06878.x.
5
Diphosphate concentration does not correlate with the level of inorganic diphosphatase in Escherichia coli.
Folia Microbiol (Praha). 1984;29(4):282-7. doi: 10.1007/BF02875958.
6
A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic pyrophosphatase.
Anal Biochem. 1981 May 15;113(2):313-7. doi: 10.1016/0003-2697(81)90082-8.
7
Accumulation of pyrophosphate correlates with the increased level of nucleoside triphosphates in Escherichia coli.
Biochem Int. 1984 Jan;8(1):1-7.
8
Magnesium transport in Escherichia coli.
J Biol Chem. 1971 Feb 10;246(3):569-76.
9
Culture medium for enterobacteria.
J Bacteriol. 1974 Sep;119(3):736-47. doi: 10.1128/jb.119.3.736-747.1974.
10
Constitutive inorganic pyrophosphatase of Escherichia coli. II. Nature and binding of active substrate and the role of magnesium.
J Biol Chem. 1966 May 10;241(9):1948-55.
Zotero 插件