在大肠杆菌K-12细胞中，细胞内焦磷酸（PPi）浓度并不直接依赖于无机焦磷酸酶的含量。
Intracellular PPi concentration is not directly dependent on amount of inorganic pyrophosphatase in Escherichia coli K-12 cells.
作者信息
Kukko-Kalske E, Lintunen M, Inen M K, Lahti R, Heinonen J
机构信息
Department of Biochemistry, University of Turku, Finland.
出版信息
J Bacteriol. 1989 Aug;171(8):4498-500. doi: 10.1128/jb.171.8.4498-4500.1989.
Abstract
No correlation was observed between the level of inorganic pyrophosphatase (PPase) and the intracellular concentration of PPi in Escherichia coli cells. In exponentially growing cells the intracellular PPi concentration was in every case 1.5 nmol/mg (dry weight) or about 0.5 mM, even though the amount of PPase was varied from 15 to 2,600% of the control amount by mutation or by using a multicopy plasmid with an inserted gene (ppa) encoding PPase. The PPi concentration could, however, be increased or decreased from the control level under some stressful conditions.
摘要
在大肠杆菌细胞中，未观察到无机焦磷酸酶（PPase）水平与细胞内焦磷酸（PPi）浓度之间存在相关性。在指数生长期的细胞中，无论通过突变或使用带有插入编码PPase基因（ppa）的多拷贝质粒，使PPase的量在对照量的15%至2600%之间变化，细胞内PPi浓度在每种情况下均为1.5 nmol/mg（干重）或约0.5 mM。然而，在某些应激条件下，PPi浓度可能会从对照水平升高或降低。
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