gdhA基因位于大肠杆菌染色体图谱的38.6分钟处。
The gdhA gene is located at 38.6 minutes on the Escherichia coli map.
作者信息
Kim S Y, McLaggan D, Epstein W
机构信息
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
出版信息
J Bacteriol. 1990 Oct;172(10):6127-8. doi: 10.1128/jb.172.10.6127-6128.1990.
Abstract
We report here that the gdhA gene of Escherichia coli, which encodes the NADP-specific glutamate dehydrogenase, is located at 38.6 min on the map. We have confirmed this location by showing linkage with three Tn10 insertions that are linked to the aroD, pheS, and ansA loci, by complementation by a restriction-mapped lambda clone, and by showing correspondence between the restriction maps of the chromosome and the cloned and sequenced gdhA gene.
摘要
我们在此报告，大肠杆菌中编码NADP特异性谷氨酸脱氢酶的gdhA基因位于染色体图谱的38.6分钟处。我们通过以下方式证实了该位置：显示其与三个与aroD、pheS和ansA位点连锁的Tn10插入片段存在连锁关系；通过一个经限制酶切图谱分析的λ克隆进行互补实验；以及显示染色体的限制酶切图谱与克隆并测序的gdhA基因之间的对应关系。
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