谷氨酸脱氢酶：产气克雷伯菌的遗传图谱绘制及调控突变体的分离
Glutamate dehydrogenase: genetic mapping and isolation of regulatory mutants of Klebsiella aerogenes.
作者信息
Bender R A, Macaluso A, Magasanik B
出版信息
J Bacteriol. 1976 Jul;127(1):141-8. doi: 10.1128/jb.127.1.141-148.1976.
Abstract
The gene for glutamate dehydrogenase (gdhD) has been mapped in Klebsiella aerogenes by P1 transduction. It is linked to pyrF and trp with the order pyrF-trp-gdh. Complementation analysis using F' episomes from Escherichia coli suggests an analogous location in E. coli. Two mutants able to produce glutamate dehydrogenase in the presence of high levels of glutamine synthetase have been isolated. One, tightly linked to gdhD, shows normal repression control by glutamine synthetase but produces four times as much glutamate dehydrogenase activity as does the wild type under all conditions tested. The other revertant is not linked to gdhD or glnA.
摘要
通过P1转导已将产气克雷伯菌中谷氨酸脱氢酶（gdhD）的基因进行了定位。它与pyrF和trp连锁，顺序为pyrF-trp-gdh。使用来自大肠杆菌的F'附加体进行的互补分析表明在大肠杆菌中有类似的定位。已分离出两个在高水平谷氨酰胺合成酶存在下能够产生谷氨酸脱氢酶的突变体。其中一个与gdhD紧密连锁，显示出受谷氨酰胺合成酶的正常阻遏控制，但在所有测试条件下产生的谷氨酸脱氢酶活性是野生型的四倍。另一个回复突变体与gdhD或glnA不连锁。
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