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1
Complete nucleotide sequence of the Escherichia coli gdhA gene.大肠杆菌gdhA基因的完整核苷酸序列。
Nucleic Acids Res. 1983 Aug 11;11(15):5257-66. doi: 10.1093/nar/11.15.5257.
2
The Klebsiella aerogenes glutamate dehydrogenase (gdhA) gene: cloning, high-level expression and hybrid enzyme formation in Escherichia coli.产气克雷伯菌谷氨酸脱氢酶（gdhA）基因：在大肠杆菌中的克隆、高水平表达及杂交酶形成
Mol Gen Genet. 1985;199(1):141-5. doi: 10.1007/BF00327523.
3
Nucleotide sequence of the promoter and amino-terminal coding region of the glutamate dehydrogenase structural gene of Escherichia coli.大肠杆菌谷氨酸脱氢酶结构基因启动子及氨基末端编码区的核苷酸序列。
Gene. 1983 Aug;23(2):199-209. doi: 10.1016/0378-1119(83)90052-5.
4
A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) in Chlorella sorokiniana.在索氏小球藻中，一个含有多个内含子的核基因编码铵诱导型叶绿体NADP特异性谷氨酸脱氢酶。
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The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa.大肠杆菌K12分支酸合酶的过表达、纯化及完整氨基酸序列，及其与粗糙脉孢菌该酶的比较。
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6
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8
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Identification of a functional promoter for the Escherichia coli gdhA gene and its regulation.大肠杆菌gdhA基因功能性启动子的鉴定及其调控
Gene. 1988 Nov 30;71(2):233-46. doi: 10.1016/0378-1119(88)90040-6.

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Plant Mol Biol. 1988 May;11(3):335-44. doi: 10.1007/BF00027390.
7
An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial NAD-Dependent Glutamate Dehydrogenase.通过定点诱变对梭菌NAD依赖型谷氨酸脱氢酶辅酶特异性测定中假定关键残基的研究。
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8
Heterologous expression of cDNAs encoding Chlorella sorokiniana NADP-specific glutamate dehydrogenase wild-type and mutant subunits in Escherichia coli cells and comparison of kinetic and thermal stability properties of their homohexamers.编码索氏小球藻NADP特异性谷氨酸脱氢酶野生型和突变亚基的cDNA在大肠杆菌细胞中的异源表达及其同型六聚体的动力学和热稳定性特性比较。
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9
Growth inhibition caused by overexpression of the structural gene for glutamate dehydrogenase (gdhA) from Klebsiella aerogenes.产气克雷伯菌谷氨酸脱氢酶结构基因（gdhA）过表达引起的生长抑制。
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10
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.

本文引用的文献

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A new computer method for the storage and manipulation of DNA gel reading data.一种用于存储和处理DNA凝胶读数数据的新型计算机方法。
Nucleic Acids Res. 1980 Aug 25;8(16):3673-94. doi: 10.1093/nar/8.16.3673.
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Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.寡脱氧核糖核苷酸的快速合成VI。通过改进的固相磷三酯路线高效、机械化合成十七聚脱氧核糖核苷酸。
Nucleic Acids Res. 1981 Apr 10;9(7):1691-706. doi: 10.1093/nar/9.7.1691.
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Codon catalog usage is a genome strategy modulated for gene expressivity.密码子编目使用是一种为基因表达性而调节的基因组策略。
Nucleic Acids Res. 1981 Jan 10;9(1):r43-74. doi: 10.1093/nar/9.1.213-b.
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Nature. 1982 Aug 19;298(5876):760-2. doi: 10.1038/298760a0.
5
Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus.嗜甲基甲基ophilus菌对甲醇转化为单细胞蛋白的转化率提高。
Nature. 1980 Oct 2;287(5781):396-401. doi: 10.1038/287396a0.
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Localisation of a strongly conserved section of coding sequence in glutamate dehydrogenase genes.谷氨酸脱氢酶基因编码序列中一个高度保守区域的定位
FEBS Lett. 1982 Oct 4;147(1):21-5. doi: 10.1016/0014-5793(82)81003-x.
7
Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.原核基因中的密码子偏好使用：最佳密码子 - 反密码子相互作用能量与高效表达基因中的选择性密码子使用
Gene. 1982 Jun;18(3):199-209. doi: 10.1016/0378-1119(82)90157-3.
8
Secondary structure predictions for the NAD-specific glutamate dehydrogenase of Neurospora crassa.粗糙脉孢菌NAD特异性谷氨酸脱氢酶的二级结构预测
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9
Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora crassa. Isolation and sequences of several cyanogen bromide peptides from the NH2-terminal portion of the peptide chain.粗糙脉孢菌的烟酰胺腺嘌呤二核苷酸特异性谷氨酸脱氢酶。肽链NH2末端部分几个溴化氰肽段的分离与序列分析。
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10
Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
Nucleic Acids Res. 1983 Apr 25;11(8):2237-55. doi: 10.1093/nar/11.8.2237.

大肠杆菌gdhA基因的完整核苷酸序列。

Complete nucleotide sequence of the Escherichia coli gdhA gene.

作者信息

McPherson M J, Wootton J C

出版信息

Nucleic Acids Res. 1983 Aug 11;11(15):5257-66. doi: 10.1093/nar/11.15.5257.

DOI:10.1093/nar/11.15.5257
PMID:6308576
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326259/
Abstract

The DNA sequence of the gdhA gene of Escherichia coli K12, which encodes the 447 amino acid polypeptide subunit of NADP-specific glutamate dehydrogenase, is presented. The deduced protein sequence is strongly homologous to the corresponding enzyme of the eukaryotic fungus Neurospora crassa. The upstream DNA sequence includes several overlapping promoter consensus sequences. The downstream DNA sequence contains inverted repeats, predicted as forming long stable stem-loop structures in RNA, homologous to those found in several enterobacterial intergenic regions.

摘要

本文展示了大肠杆菌K12的gdhA基因的DNA序列，该基因编码NADP特异性谷氨酸脱氢酶的447个氨基酸的多肽亚基。推导的蛋白质序列与真核真菌粗糙脉孢菌的相应酶具有高度同源性。上游DNA序列包含几个重叠的启动子共有序列。下游DNA序列包含反向重复序列，预测其在RNA中形成长的稳定茎环结构，与在几个肠杆菌基因间区域中发现的结构同源。