McPherson M J, Wootton J C
Nucleic Acids Res. 1983 Aug 11;11(15):5257-66. doi: 10.1093/nar/11.15.5257.
The DNA sequence of the gdhA gene of Escherichia coli K12, which encodes the 447 amino acid polypeptide subunit of NADP-specific glutamate dehydrogenase, is presented. The deduced protein sequence is strongly homologous to the corresponding enzyme of the eukaryotic fungus Neurospora crassa. The upstream DNA sequence includes several overlapping promoter consensus sequences. The downstream DNA sequence contains inverted repeats, predicted as forming long stable stem-loop structures in RNA, homologous to those found in several enterobacterial intergenic regions.
本文展示了大肠杆菌K12的gdhA基因的DNA序列,该基因编码NADP特异性谷氨酸脱氢酶的447个氨基酸的多肽亚基。推导的蛋白质序列与真核真菌粗糙脉孢菌的相应酶具有高度同源性。上游DNA序列包含几个重叠的启动子共有序列。下游DNA序列包含反向重复序列,预测其在RNA中形成长的稳定茎环结构,与在几个肠杆菌基因间区域中发现的结构同源。