College of Medical Laboratory, Dalian Medical University, Dalian 116044, China.
Atherosclerosis. 2011 Sep;218(1):151-5. doi: 10.1016/j.atherosclerosis.2011.05.024. Epub 2011 May 30.
To investigate genes associated with coronary heart disease (CHD) screened with a novel cross-validation design.
On the basis of age at the onset of the first episode of CHD, stratified sampling by age (<50 years, 50-59 years, 60-69 years, 70-79 years and >80 years) was performed. Alleles of the nine CODIS STR loci including D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, and D7S820, were determined using the STR Profiler Plus PCR amplification kit. Allele frequencies were compared with a control population. The mean age of patients with and without the alleles was compared. Cross-validation was based on differences in both frequency values and ages instead of adjustment procedure for multiple testing.
There were statistical differences in frequency values between the CHD group and the control population for three alleles, and also statistical differences in the age at first onset of CHD for two alleles; at least one allele, D21S11-28.2, was statistically different with regards to both frequency values and age. It was confirmed that D21S11-28.2 is truly related with CHD.
A single true CHD-related allele could be discriminated from the sampling errors through cross-validation. It appears that CHD-related genes may be located near to loci D21S11.
利用一种新的交叉验证设计，筛选与冠心病（CHD）相关的基因。
根据 CHD 首发年龄分层抽样（<50 岁、50-59 岁、60-69 岁、70-79 岁和>80 岁）。采用 STR Profiler Plus PCR 扩增试剂盒检测 9 个 CODIS STR 基因座（D3S1358、vWA、FGA、D8S1179、D21S11、D18S51、D5S818、D13S317 和 D7S820）的等位基因。比较患者与对照组的等位基因频率。比较有/无等位基因的患者的平均年龄。交叉验证基于频率值和年龄的差异，而不是基于多重检验的调整程序。
CHD 组与对照组在三个等位基因的频率值上存在统计学差异，在两个等位基因的 CHD 首发年龄上也存在统计学差异；至少一个等位基因 D21S11-28.2 在频率值和年龄方面均存在统计学差异。证实 D21S11-28.2 与 CHD 真正相关。
通过交叉验证，可以从抽样误差中区分出真正与 CHD 相关的等位基因。CHD 相关基因可能位于 D21S11 附近。
