School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.
J Proteomics. 2011 Sep 6;74(10):2112-22. doi: 10.1016/j.jprot.2011.05.038. Epub 2011 Jun 17.
Thermobifida fusca is an aerobic, thermophilic, cellulose degrading bacterium identified in heated organic materials. This study applied iTRAQ quantitative proteomic analysis to the cellular and membrane proteomes of T. fusca grown in presence and absence of cellulose to elucidate the cellular processes induced by cellulose nutrient. Using an iTRAQ-based quantitative proteomic approach, 783 cytosolic and 181 membrane proteins expressed during cellulose hydrolysis were quantified with ≤1% false discovery rate. The comparative iTRAQ quantification revealed considerable induction in the expression levels and up-regulation of specific proteins in cellulosic medium than non-cellulosic medium. The regulated proteins in cellulosic medium were grouped under central carbohydrate metabolism such as glycolysis/gluconeogenesis, pentose phosphate pathways, citric acid cycle, starch, sugars, pyruvate, propanoate and butanoate metabolism; energy metabolism that includes oxidative phosphorylation, nitrogen, methane and sulfur metabolism; fatty acid metabolism, amino acid metabolic pathways, purine and pyrimidine metabolism, and main cellular genetic information processing functions like replication, transcription, translation, and cell wall synthesis; and environmental information processing (membrane transport and signal transduction). The results demonstrated cellulose induced several metabolic pathways during cellulose utilization.
嗜热硬皮菌是一种好氧、嗜热、能分解纤维素的细菌，存在于加热的有机材料中。本研究采用 iTRAQ 定量蛋白质组学分析方法，研究了嗜热硬皮菌在有和没有纤维素存在的情况下的细胞和膜蛋白质组，以阐明纤维素营养物质诱导的细胞过程。使用基于 iTRAQ 的定量蛋白质组学方法，定量了 783 种细胞质和 181 种膜蛋白，假发现率≤1%。比较 iTRAQ 定量分析显示，在纤维素水解过程中，细胞内培养基中的蛋白质表达水平和特定蛋白质的上调水平明显高于非细胞内培养基。细胞内培养基中的调节蛋白被归类为中央碳水化合物代谢，如糖酵解/糖异生、戊糖磷酸途径、柠檬酸循环、淀粉、糖、丙酮酸、丙酸盐和丁酸盐代谢；能量代谢，包括氧化磷酸化、氮、甲烷和硫代谢；脂肪酸代谢、氨基酸代谢途径、嘌呤和嘧啶代谢以及主要的细胞遗传信息处理功能，如复制、转录、翻译和细胞壁合成；以及环境信息处理（膜转运和信号转导）。结果表明，纤维素在利用纤维素的过程中诱导了几种代谢途径。
