Department of Ophthalmology, People's Hospital, Peking University, Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing, China.
Am J Ophthalmol. 2011 Oct;152(4):678-685.e2. doi: 10.1016/j.ajo.2011.03.007. Epub 2011 Jun 25.
Because serum amyloid A can regulate angiogenesis, we searched for an association between serum amyloid A and interleukin-6 (IL-6), as proinflammatory factors, and proliferative diabetic retinopathy (PDR).
Retrospective, comparative study.
Seventy-six patients (76 eyes) with PDR and 31 patients (31 eyes) with nondiabetic ocular disease (control group), including idiopathic epiretinal membranes (8 eyes) and idiopathic macular holes (23 eyes), were enrolled. Enzyme-linked immunosorbent assay, dual-color immunofluorescence staining, and semiquantitative reverse-transcription polymerase chain reaction were used to examine the serum amyloid A and IL-6 levels in vitreous and plasma, expression of protein and mRNA of serum amyloid A in the excised membranes, respectively.
Vitreous serum amyloid A and IL-6 levels in the study group were significantly higher than those in the control group (both P < .001), whereas the plasma concentrations of serum amyloid A and IL-6 did not vary significantly between the groups (P = .555 and P = .621, respectively). A significant correlation was observed between the vitreous and plasma levels of serum amyloid A in subjects with PDR (r = 0.525; P < .001). In fibrovascular membranes of the study group, colocalization of endothelial marker CD31 with serum amyloid A and colocalization of fibrillar structure markers fibronectin with serum amyloid A were observed. Expression of serum amyloid A mRNA was significantly higher in fibrovascular membranes with PDR than in idiopathic epiretinal membranes (P = .004).
Serum amyloid A and IL-6 may be involved with the inflammatory process in the development of PDR. Local expression of serum amyloid A may exist in PDR.
由于血清淀粉样蛋白 A 可以调节血管生成，我们研究了其与作为促炎因子的白细胞介素-6（IL-6）之间与增生性糖尿病视网膜病变（PDR）的关系。
回顾性、对比性研究。
纳入了 76 例 PDR 患者（76 只眼）和 31 例非糖尿病眼病患者（对照组，包括特发性视网膜前膜 8 只眼和特发性黄斑裂孔 23 只眼）。采用酶联免疫吸附试验、双色免疫荧光染色和半定量逆转录聚合酶链反应分别检测玻璃体和血浆中的血清淀粉样蛋白 A 和白细胞介素-6 水平，以及切除的膜中血清淀粉样蛋白 A 蛋白和 mRNA 的表达。
研究组玻璃体中的血清淀粉样蛋白 A 和白细胞介素-6 水平明显高于对照组（均 P<0.001），而两组血浆中的血清淀粉样蛋白 A 和白细胞介素-6 浓度无显著差异（P=0.555 和 P=0.621）。PDR 患者玻璃体和血浆中的血清淀粉样蛋白 A 水平之间存在显著相关性（r=0.525；P<0.001）。在研究组的纤维血管膜中，内皮标志物 CD31 与血清淀粉样蛋白 A 共定位，纤维状结构标志物纤维连接蛋白与血清淀粉样蛋白 A 共定位。PDR 纤维血管膜中血清淀粉样蛋白 A mRNA 的表达明显高于特发性视网膜前膜（P=0.004）。
血清淀粉样蛋白 A 和白细胞介素-6 可能参与了 PDR 发展过程中的炎症反应。局部表达的血清淀粉样蛋白 A 可能存在于 PDR 中。
