Department of Ophthalmology, Boston University School of Medicine & Boston Medical Center, Boston, MA 02118, USA.
Department of Medicine (Biomedical Genetics), Boston University School of Medicine, 72 East Concord Street, Boston, MA 02118, USA.
Cells. 2021 Apr 30;10(5):1069. doi: 10.3390/cells10051069.
In this study, we compare the vitreous cytokine profile in patients with proliferative diabetic retinopathy (PDR) to that of patients without PDR. The identification of novel cytokines involved in the pathogenesis of PDR provides candidate therapeutic targets that may stand alone or work synergistically with current therapies in the management of diabetic retinopathy. Undiluted vitreous humor specimens were collected from 74 patients undergoing vitrectomy for various vitreoretinal disorders. Quantitative immunoassay was performed for a panel of 36 neuroinflammatory cytokines in each specimen and assessed to identify differences between PDR ( = 35) and non-PDR ( = 39) patients. Levels of interleukin-8 (IL-8), IL-15, IL-16, vascular endothelial growth factor (VEGF), VEGF-D, c-reactive protein (CRP), serum amyloid-A (SAA), and intracellular adhesion molecule-1 (ICAM1) were significantly increased in the vitreous of PDR patients compared to non-PDR patients ( < 0.05). We report novel increases in IL-15 and IL-16, in addition to the expected VEGF, in the human vitreous humor of patients with PDR. Additionally, we confirm the elevation of ICAM-1, VCAM-1, SAA, IL-8 and CRP in the vitreous of patients with PDR, which has previously been described.
在这项研究中，我们比较了增生性糖尿病视网膜病变（PDR）患者和无 PDR 患者的玻璃体细胞因子谱。鉴定参与 PDR 发病机制的新型细胞因子为糖尿病性视网膜病变的治疗提供了候选靶点，这些靶点可能单独或与当前的治疗方法协同作用。从 74 名因各种玻璃体视网膜疾病接受玻璃体切除术的患者中收集未稀释的玻璃体标本。对每个标本进行了 36 种神经炎症细胞因子的定量免疫分析，并评估了其差异，以确定 PDR（=35）和非 PDR（=39）患者之间的差异。与非 PDR 患者相比，PDR 患者的玻璃体中白细胞介素-8（IL-8）、白细胞介素-15（IL-15）、白细胞介素-16（IL-16）、血管内皮生长因子（VEGF）、VEGF-D、C 反应蛋白（CRP）、血清淀粉样蛋白-A（SAA）和细胞间黏附分子-1（ICAM1）水平明显升高（<0.05）。除了预期的 VEGF 外，我们还报告了 PDR 患者玻璃体中 IL-15 和 IL-16 的新增加。此外，我们还证实了 PDR 患者玻璃体中 ICAM-1、VCAM-1、SAA、IL-8 和 CRP 的升高，这以前已有描述。
