Unit of Toxicology, CURML, 1 rue Michel-Servet, 1211 Geneva 4, Switzerland.
Anal Bioanal Chem. 2012 Mar;402(8):2485-98. doi: 10.1007/s00216-011-5161-6. Epub 2011 Jun 25.
Because of the emergence of dried blood spots (DBS) as an attractive alternative to conventional venous plasma sampling in many pharmaceutical companies and clinical laboratories, different analytical approaches have been developed to enable automated handling of DBS samples without any pretreatment. Associated with selective and sensitive MS-MS detection, these procedures give good results in the rapid identification and quantification of drugs (generally less than 3 min total run time), which is desirable because of the high throughput requirements of analytical laboratories. The objective of this review is to describe the analytical concepts of current direct DBS techniques and to present their advantages and disadvantages, with particular focus on automation capacity and commercial availability. Finally, an overview of the different biomedical applications in which these concepts could be of major interest will be presented.
由于干血斑（DBS）作为传统静脉血浆采样的一种有吸引力的替代品，在许多制药公司和临床实验室中出现，因此已经开发了不同的分析方法，以便能够在无需任何预处理的情况下自动处理 DBS 样本。与选择性和灵敏的 MS-MS 检测相关，这些程序在快速鉴定和定量药物方面取得了良好的结果（通常总运行时间少于 3 分钟），这是因为分析实验室对高通量的要求很高。本综述的目的是描述当前直接 DBS 技术的分析概念，并介绍它们的优缺点，特别关注自动化能力和商业可用性。最后，将介绍这些概念可能具有重要意义的不同生物医学应用的概述。
