Laboratório de Genética, Departamento de Genética e Morfologia, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, DF, Brazil.
Eur J Appl Physiol. 2012 Mar;112(3):941-50. doi: 10.1007/s00421-011-1961-9. Epub 2011 Jun 26.
Physical training induces beneficial adaptations, but exhausting exercise increases reactive oxygen species, which can cause muscular injuries with consequent inflammatory processes, implying jeopardized performance and possibly overtraining. Acute strenuous exercise almost certainly exceeds the benefits of physical activity; it can compromise performance and may contribute to increased future risk of cardiovascular disease (CVD) in athletes. Polymorphisms in the muscle-type creatine kinase (CK-MM) gene may influence performance and adaptation to training, while many potentially significant genetic variants are reported as risk factors for CVD. Therefore, we investigated the influence of polymorphisms in CK-MM TaqI and NcoI, methylenetetrahydrofolate reductase (MTHFR C677T and A1298C) and C-reactive protein (CRP G1059C) genes on exercise-induced damage and inflammation markers. Blood samples were taken immediately after a race (of at least 4 km) that took place outdoors on flat tracks, and were submitted to genotyping and biochemical evaluation of aspartate aminotransferase (AST), CK, CRP and high-sensitivity CRP (hs-CRP). CK-MM TaqI polymorphism significantly influenced results of AST, CK and hs-CRP, and an association between MTHFR C677T and A1298C with CRP level was found, although these levels did not exceed reference values. The results indicate that these polymorphisms can indirectly influence performance, contribute to higher susceptibility to exercise-induced inflammation or protection against it, and perhaps affect future risks of CVD in athletes.
体能训练可引起有益的适应性变化，但过度的运动可增加活性氧物种，从而导致肌肉损伤和随后的炎症过程，这意味着运动表现受损，并且可能导致过度训练。剧烈的急性运动几乎肯定超过了身体活动的益处；它会损害运动表现，并可能导致运动员心血管疾病（CVD）的未来风险增加。肌肉型肌酸激酶（CK-MM）基因的多态性可能会影响运动表现和对训练的适应，而许多潜在的重要遗传变异被报道为 CVD 的危险因素。因此，我们研究了 CK-MM TaqI 和 NcoI、亚甲基四氢叶酸还原酶（MTHFR C677T 和 A1298C）和 C-反应蛋白（CRP G1059C）基因多态性对运动引起的损伤和炎症标志物的影响。在户外平坦赛道上进行的至少 4 公里的比赛后，立即采集血样，并进行基因分型和天冬氨酸氨基转移酶（AST）、CK、CRP 和高敏 CRP（hs-CRP）的生化评估。CK-MM TaqI 多态性显著影响 AST、CK 和 hs-CRP 的结果，并且发现 MTHFR C677T 和 A1298C 与 CRP 水平之间存在关联，尽管这些水平未超过参考值。结果表明，这些多态性可以间接影响运动表现，增加对运动引起的炎症的易感性或对其的保护作用，并且可能影响运动员未来的 CVD 风险。
