Functional Plant Biology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India.
Mol Biol Rep. 2012 Mar;39(3):3311-8. doi: 10.1007/s11033-011-1100-z. Epub 2011 Jun 26.
Semi-quantitative RT-PCR based transcript expression of stress responsive genes was studied in leaves of sugarcane plants exposed to short-term (up to 24 h) salt (NaCl, 200 mM) or polyethylene glycol-PEG 8000 (20% w/v) stress. Transient increase in expression of NHX (sodium proton antiporter), SUT1 (sucrose transporter1), PDH (proline dhydrogenase) and CAT2 (catalase2) was observed in response to 2-4 h PEG stress. However, salt stress imposed repression of NHX, PDH and CAT2 at these time points. The transcript level of the delta (1)-pyrolline-5-carboxylate synthetase (P5CS) increased slightly in salt treatment while in response to the PEG stress, the gene expression increased at 4 h treatment but then decreased considerably by 80% at 24 h. The results thus indicated differential regulation of these stress responsive genes in response to salt or PEG stress in sugarcane. Further, the transcript expression data was compared with that available for the Arabidopsis homologs at Arabidopsis eFP Browser and Genevestigator V3 tools. Understanding transcript gene expression patterns of the stress responsive genes may provide insights into complex regulatory network of stress tolerance.
基于半定量 RT-PCR 的研究表明，在短期（长达 24 小时）盐（NaCl，200mM）或聚乙二醇-PEG8000（20％w/v）胁迫下，甘蔗叶片中应激响应基因的转录表达。在 PEG 胁迫 2-4 小时时，观察到 NHX（钠离子质子反向转运蛋白）、SUT1（蔗糖转运蛋白 1）、PDH（脯氨酸脱氢酶）和 CAT2（过氧化氢酶 2）的表达短暂增加。然而，在这些时间点，盐胁迫抑制了 NHX、PDH 和 CAT2 的表达。在盐处理中，delta（1）-吡咯啉-5-羧酸合成酶（P5CS）的转录水平略有增加，而在 PEG 胁迫下，基因表达在 4 小时处理时增加，但在 24 小时处理时却减少了 80%。因此，这些结果表明，在甘蔗中，这些应激响应基因对盐或 PEG 胁迫的反应存在差异调节。此外，将转录表达数据与 Arabidopsis eFP Browser 和 Genevestigator V3 工具中拟南芥同源物的转录表达数据进行比较。了解应激响应基因的转录表达模式可能有助于深入了解应激耐受的复杂调控网络。
