Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil.
Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos dos Goytacazes, RJ, Brazil.
PLoS One. 2017 Apr 18;12(4):e0176076. doi: 10.1371/journal.pone.0176076. eCollection 2017.
Salt stress is one of the most common stresses in agricultural regions worldwide. In particular, sugarcane is affected by salt stress conditions, and no sugarcane cultivar presently show high productivity accompanied by a tolerance to salt stress. Proteomic analysis allows elucidation of the important pathways involved in responses to various abiotic stresses at the biochemical and molecular levels. Thus, this study aimed to analyse the proteomic effects of salt stress in micropropagated shoots of two sugarcane cultivars (CB38-22 and RB855536) using a label-free proteomic approach. The mass spectrometry proteomics data are available via ProteomeXchange with identifier PXD006075. The RB855536 cultivar is more tolerant to salt stress than CB38-22. A quantitative label-free shotgun proteomic analysis identified 1172 non-redundant proteins, and 1160 of these were observed in both cultivars in the presence or absence of NaCl. Compared with CB38-22, the RB855536 cultivar showed a greater abundance of proteins involved in non-enzymatic antioxidant mechanisms, ion transport, and photosynthesis. Some proteins, such as calcium-dependent protein kinase, photosystem I, phospholipase D, and glyceraldehyde-3-phosphate dehydrogenase, were more abundant in the RB855536 cultivar under salt stress. Our results provide new insights into the response of sugarcane to salt stress, and the changes in the abundance of these proteins might be important for the acquisition of ionic and osmotic homeostasis during exposure to salt stress.
盐胁迫是全球农业地区最常见的胁迫之一。特别是，甘蔗受到盐胁迫的影响，目前没有任何甘蔗品种表现出高产的同时还能耐受盐胁迫。蛋白质组学分析可以阐明在生化和分子水平上应对各种非生物胁迫的重要途径。因此，本研究旨在使用无标记蛋白质组学方法分析微繁殖甘蔗品种（CB38-22 和 RB855536）对盐胁迫的蛋白质组效应。质谱蛋白质组学数据可通过 ProteomeXchange 以标识符 PXD006075 获得。RB855536 品种比 CB38-22 更能耐受盐胁迫。定量无标记 shotgun 蛋白质组学分析鉴定了 1172 个非冗余蛋白质，其中 1160 个在两种品种中均存在于有无 NaCl 的情况下。与 CB38-22 相比，RB855536 品种中非酶抗氧化机制、离子转运和光合作用中涉及的蛋白质丰度更高。一些蛋白质，如钙依赖性蛋白激酶、光系统 I、磷脂酶 D 和甘油醛-3-磷酸脱氢酶，在 RB855536 品种在盐胁迫下更丰富。我们的研究结果为甘蔗对盐胁迫的响应提供了新的见解，这些蛋白质丰度的变化可能对在盐胁迫下获得离子和渗透平衡很重要。
