Joshi B, Rundell K
Department of Microbiology-Immunology, Northwestern University, Chicago, Illinois 60611.
J Virol. 1990 Nov;64(11):5649-51. doi: 10.1128/JVI.64.11.5649-5651.1990.
Two cellular proteins, 61 and 37 kDa, are found in association with the simian virus 40 (SV40) small-t antigen. Fractionation in standard chromatography systems showed that these proteins were associated with one another in uninfected cells, suggesting that the small-t antigen may bind the complex as a whole and not each individual protein independently. In the presence of N-ethylmaleimide, the 37-kDA protein was selectively released from immune complexes, leaving the small-t antigen and 61-kDa protein in association. This result suggests that the small-t antigen may bind only the 61-kDa protein and that the 37-kDa protein may be associated with immune complexes by virtue of its association with the 61-kDa cellular protein.
发现两种细胞蛋白，分子量分别为61 kDa和37 kDa，与猴病毒40（SV40）小t抗原相关联。在标准色谱系统中进行分级分离显示，这些蛋白在未感染的细胞中彼此相关联，这表明小t抗原可能作为一个整体结合该复合物，而不是分别独立地结合每个蛋白质。在存在N - 乙基马来酰亚胺的情况下，37 kDa蛋白从免疫复合物中被选择性释放，留下小t抗原和61 kDa蛋白仍保持关联。这一结果表明，小t抗原可能仅结合61 kDa蛋白，而37 kDa蛋白可能因其与61 kDa细胞蛋白的关联而与免疫复合物相关联。
