Phillips B, Rundell K
Cancer Center, Medical School, Northwestern University, Chicago, Illinois 60611.
J Virol. 1988 Mar;62(3):768-75. doi: 10.1128/JVI.62.3.768-775.1988.
Mouse C3H 10T1/2 cell lines expressing the simian virus 40 (SV40) small t antigen were obtained by cotransfection of pSV2neo and plasmids which encode small t. Cell lines derived from two plasmids which encode small t in the absence of stable deletion fragments of the large T antigen were morphologically normal and grew to slightly higher saturation densities in low serum than control cell lines. Unexpectedly, the clones had highly organized actin cables, as did parental 10T1/2 cells infected with wild-type SV40. These observations and comparisons of rat F111 cells infected with either polyomavirus or SV40 suggest that the SV40 small t antigen does not directly affect cytoskeletal organization.
通过共转染pSV2neo和编码小t抗原的质粒，获得了表达猿猴病毒40（SV40）小t抗原的小鼠C3H 10T1/2细胞系。来自两个编码小t抗原的质粒且不存在大T抗原稳定缺失片段的细胞系，其形态正常，在低血清条件下生长至饱和密度时比对照细胞系略高。出乎意料的是，这些克隆具有高度有序的肌动蛋白丝束，感染野生型SV40的亲代10T1/2细胞也是如此。这些观察结果以及对感染多瘤病毒或SV40的大鼠F111细胞的比较表明，SV40小t抗原不会直接影响细胞骨架的组织。
