State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Marine Pharmacology & Toxicology Lab, Department of Marine Science, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India.
Appl Biochem Biotechnol. 2024 Jul;196(7):3914-3948. doi: 10.1007/s12010-023-04738-w. Epub 2023 Oct 4.
In this study, the anti-biofilm compound of 2,6-Di-tert-butyl, 1,4-benzoquinone was purified from Nocardiopsis synnemataformans (N. synnemataformans) RMN 4 (MN061002). To confirm the compound, various spectroscopy analyses were done including ultraviolet (UV) spectrometer, Fourier transform infrared spectroscopy (FTIR), analytical high-performance liquid chromatography (HPLC), preparative HPLC, gas chromatography-mass spectroscopy (GC-MS), liquid chromatography-mass spectroscopy (LC-MS), and 2D nuclear magnetic resonance (NMR). Furthermore, the purified compound was shown 94% inhibition against biofilm-producing Proteus mirabilis (P. mirabilis) (MN396686) at 70 µg/mL concentrations. Furthermore, the metabolic activity, exopolysaccharide damage, and hydrophobicity degradation results of identified compound exhibited excellent inhibition at 100 µg/mL concentration. Furthermore, the confocal laser scanning electron microscope (CLSM) and scanning electron microscope (SEM) results were shown with intracellular damages and architectural changes in bacteria. Consecutively, the in vivo toxicity effect of the compound against Artemia franciscana (A. franciscana) was shown to have a low mortality rate at 100 µg/mL. Finally, the molecular docking interaction between the quorum sensing (QS) genes and identified compound clearly suggested that the identified compound 2,6-Di-tert-butyl, 1,4-benzoquinone has anti-quorum sensing and anti-biofilm activities against P. mirabilis (MN396686).
在这项研究中，从链霉菌（Nocardiopsis synnemataformans）RMN 4（MN061002）中纯化出了 2,6-二叔丁基-1,4-苯醌这种抗生物膜化合物。为了确认该化合物，我们进行了各种光谱分析，包括紫外（UV）分光光度计、傅里叶变换红外光谱（FTIR）、分析高效液相色谱（HPLC）、制备高效液相色谱、气相色谱-质谱（GC-MS）、液相色谱-质谱（LC-MS）和二维核磁共振（NMR）。此外，该纯化化合物在 70μg/mL 浓度下对产生物膜的奇异变形菌（Proteus mirabilis）（MN396686）表现出 94%的抑制作用。此外，在 100μg/mL 浓度下，鉴定出的化合物对代谢活性、胞外多糖损伤和疏水性降解的结果表现出极好的抑制作用。此外，共聚焦激光扫描电子显微镜（CLSM）和扫描电子显微镜（SEM）结果显示，细菌的细胞内损伤和结构发生了变化。接着，化合物对卤虫（Artemia franciscana）的体内毒性作用表明，在 100μg/mL 浓度下死亡率较低。最后，群体感应（QS）基因与鉴定出的化合物之间的分子对接相互作用清楚地表明，鉴定出的化合物 2,6-二叔丁基-1,4-苯醌对奇异变形菌（MN396686）具有抗群体感应和抗生物膜活性。
