Department of Chemical and Process Engineering G.B. Bonino, University of Genoa, Via Opera Pia 15, 16145 Genoa, Italy.
Nat Prod Res. 2011 Oct;25(18):1751-61. doi: 10.1080/14786419.2010.524889. Epub 2011 Jun 27.
The aim of this research was to study the recovery of phenolic compounds from grape seeds, by-products from winemaking industries, using ethanolic solid-liquid extraction. For such a purpose, the combined effects of the extraction time (9, 19 and 29 h) and the solid-liquid ratio (0.10, 0.20 and 0.30 gdw mL(-1)), were investigated (where dw = dry waste). Results demonstrated that Pinot Noir seeds had high levels of both total polyphenols (73.66 mg(Gallic Acid Equivalent) gdw(-1)) and flavonoids (30.90 mg(Catechin Equivalent) gdw(-1)), being the optimum extraction time 19 h approximately. The main phenolic compounds analysed with high performance liquid chromatography were catechin and quercetin with a maximum extraction yield obtained at 29 h (362.23 and 339.35 mg/100 gdw, respectively). Concentration of the polyphenols and their antiradical powers are demonstrated to have a significant linear correlation.
本研究旨在利用乙醇固液萃取法，从酿酒工业的副产物——葡萄种子中提取酚类化合物。为此，考察了提取时间（9、19 和 29 小时）和固液比（0.10、0.20 和 0.30 gdw/mL）的联合作用（其中 dw=干废物）。结果表明，黑比诺种子的总多酚（73.66 mg（没食子酸当量）/gdw（-1））和类黄酮（30.90 mg（儿茶素当量）/gdw（-1））含量均较高，最佳提取时间约为 19 小时。用高效液相色谱法分析的主要酚类化合物为儿茶素和槲皮素，在 29 小时时获得最大提取产率（分别为 362.23 和 339.35 mg/100 gdw）。多酚的浓度及其抗氧化能力呈显著线性相关。
