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HPLC-tandem mass spectrometric method to characterize resveratrol metabolism in humans.用于表征白藜芦醇在人体内代谢情况的高效液相色谱-串联质谱法。
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Kaempferol in red and pinto bean seed (Phaseolus vulgaris L.) coats inhibits iron bioavailability using an in vitro digestion/human Caco-2 cell model.红芸豆和斑豆种子(菜豆)种皮中的山奈酚通过体外消化/人Caco-2细胞模型抑制铁的生物利用率。
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Cocoa-related flavonoids inhibit CFTR-mediated chloride transport across T84 human colon epithelia.可可相关的类黄酮抑制CFTR介导的氯离子跨T84人结肠上皮细胞的转运。
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Application of liquid chromatography/electrospray ionization tandem mass spectrometry to the analysis of polyphenolic compounds from an infusion of Byrsonima crassa Niedenzu.液相色谱/电喷雾电离串联质谱法在分析厚叶比耶森木(Byrsonima crassa Niedenzu)浸出液中多酚类化合物的应用
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利用液相色谱-紫外/可见光谱法(LC-UV/vis)、液相色谱-串联质谱法(LC-MS/MS)对人心果‘蒙哥马利’果实中的酚类物质进行表征,并利用气相色谱法(GC)对糖类物质进行表征。

Characterization of phenolics by LC-UV/vis, LC-MS/MS and sugars by GC in Melicoccus bijugatus Jacq. 'Montgomery' fruits.

作者信息

Bystrom Laura M, Lewis Betty A, Brown Dan L, Rodriguez Eloy, Obendorf Ralph L

机构信息

Division of Nutritional Sciences, Cornell University, Ithaca, NY, USA.

出版信息

Food Chem. 2008 Dec 15;111(4):1017-1024. doi: 10.1016/j.foodchem.2008.04.058.

DOI:10.1016/j.foodchem.2008.04.058
PMID:21709744
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3123376/
Abstract

Fruits of the native South American tree Melicoccus bijugatus Jacq. (Sapindaceae) are consumed for both dietary and medicinal purposes, but limited information is available about the phytochemistry and health value of M. bijugatus fruits. Fruit tissues of the Florida Montgomery cultivar were assessed for sugars, using gas chromatography, and for total phenolics, using UV spectroscopy. Reverse phase high performance liquid chromatography (HPLC) fingerprints of crude methanolic pulp, embryo and seed coat extracts were obtained at 280 nm. Phenolics were characterised by both HPLC UV/vis analysis and HPLC electrospray ionization tandem mass spectrometry. Major sugars detected in the pulp and embryo extracts were sucrose, followed by glucose and fructose. The glucose:fructose ratio was 1:1 in the pulp and 0.1:1 in the embryo. Total phenolic concentrations of the fruit tissues were in the order: seed coat > embryo > pulp. Phenolic acids were identified mostly in pulp tissues. Phenolic acids, flavonoids, procyanidins and catechins were identified in embryo tissues, and higher molecular weight procyanidins were identified in seed coat tissues. This study provides new information about the phytochemistry and the potential health value of the Montgomery cultivar M. bijugatus fruit tissues.

摘要

南美洲本土树木双珠母杨梅(Melicoccus bijugatus Jacq.,无患子科)的果实可用于饮食和药用,但关于双珠母杨梅果实的植物化学和健康价值的信息有限。使用气相色谱法评估了佛罗里达蒙哥马利品种果实组织中的糖分,并使用紫外光谱法评估了总酚含量。在280nm处获得了粗甲醇果肉、胚和种皮提取物的反相高效液相色谱(HPLC)指纹图谱。通过HPLC紫外/可见分析和HPLC电喷雾电离串联质谱对酚类进行了表征。果肉和胚提取物中检测到的主要糖类为蔗糖,其次是葡萄糖和果糖。果肉中葡萄糖与果糖的比例为1:1,胚中为0.1:1。果实组织的总酚浓度顺序为:种皮>胚>果肉。酚酸主要在果肉组织中被鉴定出来。胚组织中鉴定出了酚酸、黄酮类、原花青素和儿茶素,种皮组织中鉴定出了分子量更高的原花青素。本研究提供了有关蒙哥马利品种双珠母杨梅果实组织的植物化学和潜在健康价值的新信息。