Bache Michael, Taboryski Rafael, Schmid Silvan, Aamand Jens, Jakobsen Mogens Havsteen
Department of Micro- and Nanotechnology, Technical University of Denmark, DTU Nanotech, Building 345 East, 2800 Kongens Lyngby, Denmark.
Nanoscale Res Lett. 2011 May 16;6(1):386. doi: 10.1186/1556-276X-6-386.
The attachment of an antibody to an antigen-coated cantilever has been investigated by repeated experiments, using a cantilever-based detection system by Cantion A/S. The stress induced by the binding of a pesticide residue BAM (2,6 dichlorobenzamide) immobilized on a cantilever surface to anti-BAM antibody is measured using the CantiLab4© system from Cantion A/S with four gold-coated cantilevers and piezo resistive readout. The detection mechanism is in principle label-free, but fluorescent-marked antibodies have been used to subsequently verify the binding on the cantilever surface. The bending and increase in mass of each cantilever has also been investigated using a light interferometer and a Doppler Vibrometer. The system has been analyzed during repeated measurements to investigate whether the CantiLab4© system is a suited platform for a pesticide assay system.
通过重复实验，利用丹麦坎蒂昂公司（CANTION A/S）基于悬臂梁的检测系统，对抗体与抗原包被悬臂梁的结合情况进行了研究。使用丹麦坎蒂昂公司的CantiLab4©系统，通过四个镀金悬臂梁和压阻式读出装置，测量固定在悬臂梁表面的农药残留BAM（2,6 - 二氯苯甲酰胺）与抗BAM抗体结合所诱导的应力。检测机制原则上是无标记的，但已使用荧光标记抗体来随后验证在悬臂梁表面的结合情况。还使用光干涉仪和多普勒振动计研究了每个悬臂梁的弯曲和质量增加情况。在重复测量过程中对该系统进行了分析，以研究CantiLab4©系统是否是农药检测系统的合适平台。
