Bhanot Abhishek, Shri Richa
Amar Shaheed Baba Ajit Singh Jujhar Singh Memorial (ASBASJSM) College of Pharmacy, BELA, Ropar - 1401 11, Punjab, India.
Pharmacognosy Res. 2010 Nov;2(6):374-84. doi: 10.4103/0974-8490.75460.
Diabetic Neuropathy (DN) is a major microvascular complication of uncontrolled diabetes. This may result from increased oxidative stress that accompanies diabetes. Hence plants with antioxidant action play an important role in management of diabetes and its complications.
This study was designed to evaluate preventive as well as curative effect of methanol extracts of outer scales and edible portions of two plants with established antioxidant action - Allium cepa and Allium sativum, in induced DN in albino mice. Mice were divided into control, diabetic and test extracts treated groups. Test extracts were administered daily at a dose of 200 mg/kg p.o. for 21 days, in the preventive group prior to onset of DN, and in the curative group after the onset of DN. Hyperalgesia and oxidative stress markers were assessed. STZ-diabetic mice showed a significant thermal hyperalgesia (as assessed by the tail-flick test), indicating development of DN.
Treatment with test extracts prevented loss in body weight, decreased plasma glucose level, and significantly ameliorated the hyperalgesia, TBARS, serum nitrite and GSH levels in diabetic mice.
Methanol extract of outer scales of onion has shown most significant improvement; may be due to higher content of phenolic compounds in outer scales of A. cepa.
糖尿病神经病变（DN）是未控制的糖尿病的一种主要微血管并发症。这可能是由糖尿病伴随的氧化应激增加所致。因此，具有抗氧化作用的植物在糖尿病及其并发症的管理中发挥着重要作用。
本研究旨在评估两种具有已确定抗氧化作用的植物——洋葱和大蒜的外层鳞片及可食用部分的甲醇提取物对白化病小鼠诱导性DN的预防和治疗效果。将小鼠分为对照组、糖尿病组和试验提取物治疗组。试验提取物以200mg/kg口服给药，每日一次，共21天，在预防组于DN发病前给药，在治疗组于DN发病后给药。评估痛觉过敏和氧化应激标志物。链脲佐菌素诱导的糖尿病小鼠表现出显著的热痛觉过敏（通过甩尾试验评估），表明DN的发展。
试验提取物治疗可防止体重减轻，降低血糖水平，并显著改善糖尿病小鼠的痛觉过敏、硫代巴比妥酸反应物（TBARS）、血清亚硝酸盐和谷胱甘肽（GSH）水平。
洋葱外层鳞片的甲醇提取物显示出最显著的改善；这可能是由于洋葱外层鳞片中酚类化合物含量较高。
