Department of Biological Engineering, University of Missouri, Columbia, Missouri, USA.
J Biomed Mater Res B Appl Biomater. 2011 Oct;99(1):142-9. doi: 10.1002/jbm.b.31881. Epub 2011 Jun 28.
Polyethylene terephthalate (PET) mesh is one of the most commonly used synthetic biomaterials for tension-free hernia repair. In an effort to improve the biocompatibility of PET mesh, gold nanoparticles (AuNP) in various concentrations were conjugated to the PET surface to develop PET-AuNP scaffolds. These novel scaffolds were characterized with Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC) to assess the addition of functional groups, presence of AuNPs, and thermal stability of the modified PET mesh, respectively. The biocompatibility of the PET-AuNP scaffolds was evaluated through in vitro cell culture assays. The cellularity of cells exposed to the PET-AuNP scaffolds, as well as the scaffolds' ability to reduce reactive oxygen species, was assessed using L929 murine fibroblasts. Antimicrobial properties of AuNPs conjugated to PET mesh were tested against the bacteria Pseudomonas aeruginosa. Results from the FT-IR showed presence of COOH groups while SEM displayed bonding of AuNPs to the PET surface. DSC results indicated that the PET more than likely did not undergo any detrimental degradation due to the surface modification. Results from the in vitro studies showed that AuNPs, in optimal concentrations (1× concentrations), enhanced cellularity, reduced ROS, and reduced bacteria adhesion to PET. These studies demonstrated enhanced biocompatibility of the AuNP conjugated PET mesh over pristine PET mesh.
聚对苯二甲酸乙二醇酯（PET）网是用于无张力疝修补的最常用的合成生物材料之一。为了提高 PET 网的生物相容性，用不同浓度的金纳米粒子（AuNP）修饰 PET 表面，制备了 PET-AuNP 支架。通过傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）和差示扫描量热法（DSC）分别对这些新型支架进行了功能基团的添加、AuNPs 的存在和改性 PET 网的热稳定性进行了表征。通过体外细胞培养实验评估了 PET-AuNP 支架的生物相容性。使用 L929 鼠成纤维细胞评估了暴露于 PET-AuNP 支架的细胞的细胞活力以及支架减少活性氧（ROS）的能力。还测试了与 PET 网结合的 AuNPs 对铜绿假单胞菌的抗菌性能。FT-IR 的结果显示存在 COOH 基团，而 SEM 则显示 AuNPs 与 PET 表面的结合。DSC 结果表明，由于表面修饰，PET 极不可能发生任何有害降解。体外研究的结果表明，在最佳浓度（1×浓度）下，AuNPs 增强了细胞活力、减少了 ROS，并减少了细菌对 PET 的黏附。这些研究表明，AuNP 修饰的 PET 网比原始的 PET 网具有更好的生物相容性。
