Urazova L N, Kuznetsova T I, Boev R S, Burkova V N
Cancer Research Institute, Siberian Branch of the Russian Academy of Medical Sciences, Tomsk, Russia.
Exp Oncol. 2011 Jun;33(2):90-3.
To study the influence of natural L-asparagine on the efficacy of cytostatic therapy for malignant tumors in experimental investigations.
Female C57B1/6 mice weighing 18-20 g were selected for the experiments. Lewis' lung carcinoma (LLC) and melanoma B16 cells were used in the study. Animals were inoculated with tumor cells intramuscularly. Solution of L-asparagine in a volume of 0.2 ml per mouse (in appropriate doses) was administered to the animals using gastric probe, daily, for 14 days. Cyclophosphane was administered intraperitoneally in total doses of 180 mg/kg and 90 mg/kg on days 3 and 7 after tumor implantation. The percentage of tumor growth inhibition was calculated and inhibition index and frequency of metastasis were assessed.
It has been shown that despite low activity of L-asparagine with regard to primary tumor, the level of metastasis inhibition is rather high (up to 91% depending on experimental model, therapy regimen and follow-up period). The analysis of previously obtained data and our studies indicate that L-asparagine derived from burdock (Arctium lappa) root has not only its own antimetastatic activity but it is also able to increase antimetastatic activity of cyclophosphane partially reducing toxic effect of cyclophosphane on the organism without decreasing its antitumor and antimetastatic activities.
L-asparagine derived from burdock (Arctium lappa) root can be effective in the complex anticancer therapy with the use of appropriate chemotherapy doses and regimens.
在实验研究中探讨天然L-天冬酰胺对恶性肿瘤细胞抑制疗法疗效的影响。
选用体重18 - 20 g的雌性C57B1/6小鼠进行实验。研究中使用Lewis肺癌（LLC）和黑色素瘤B16细胞。动物通过肌肉注射接种肿瘤细胞。使用胃管给动物每日注射每只0.2 ml的L-天冬酰胺溶液（适当剂量），持续14天。在肿瘤植入后的第3天和第7天，腹腔注射环磷酰胺，总剂量分别为180 mg/kg和90 mg/kg。计算肿瘤生长抑制百分比，并评估抑制指数和转移频率。
结果表明，尽管L-天冬酰胺对原发性肿瘤的活性较低，但转移抑制水平相当高（根据实验模型、治疗方案和随访期，高达91%）。对先前获得的数据和我们的研究分析表明，牛蒡根中的L-天冬酰胺不仅具有自身的抗转移活性，还能够增强环磷酰胺的抗转移活性，部分降低环磷酰胺对机体的毒性作用，同时不降低其抗肿瘤和抗转移活性。
牛蒡根中的L-天冬酰胺在采用适当化疗剂量和方案的综合抗癌治疗中可能有效。
