École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.
Opt Lett. 2010 Dec 15;35(24):4102-4. doi: 10.1364/OL.35.004102.
We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.
我们之前开发了一种用于非扫描二次谐波产生（SHG）显微镜的新方法[Opt. Lett. 34, 2450 (2009)]。基于数字全息术，该技术在单次全息图采集过程中同时捕获相干 SHG 辐射的振幅和相位，从而实现了二次谐波相位显微镜。在这项工作中，我们展示了无标记生物组织的全息 SHG 相位显微镜，并讨论了其对 SHG 显微镜的附加价值。
