Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Sciences, Ministry of Justice, Shanghai, China.
Int J Legal Med. 2011 Sep;125(5):753-6. doi: 10.1007/s00414-011-0592-3. Epub 2011 Jun 30.
X-chromosomal short tandem repeats (X-STRs) loci are used for forensic practice in recent years which play increasingly important roles in some complex kinship cases. In this paper, a new multiplex polymerase chain reaction (PCR) system which can simultaneously analyze 11 X-STR markers (DXS8378, DXS6795, DXS7132, DXS6803, DXS9898, DXS6801, DXS7133, GATA165B12, HPRTB, DXS8377 and DXS7423) was developed. The samples of 1,605 (742 males and 863 females) unrelated individuals from Tibetan and Northern Han population were successfully analyzed using this multiplex system. A total of 103 alleles for all the loci were observed. Hardy-Weinberg equilibrium tests demonstrated no significant deviation from expected values (P > 0.05) for all of the 11 X-STR loci in the two studied populations. Polymorphism information contents of the loci were 0.3864-0.9013, and powers of discrimination in females of the loci were 0.6317-0.9845. There were no statistically significant differences between Tibetan and Northern Han populations in allele distribution of the 11 X-STR loci, in line with analysis of molecular variance (AMOVA) results. Our work indicates that this multiplex system is useful for forensic analysis for the two populations in China.
X 染色体短串联重复序列（X-STRs）位点近年来在法医实践中得到了广泛应用，在一些复杂的亲属关系案件中发挥着越来越重要的作用。本文开发了一种新的多重聚合酶链反应（PCR）系统，可同时分析 11 个 X-STR 标记（DXS8378、DXS6795、DXS7132、DXS6803、DXS9898、DXS6801、DXS7133、GATA165B12、HPRTB、DXS8377 和 DXS7423）。利用该多重系统成功分析了来自藏族和北方汉族的 1605 名（742 名男性和 863 名女性）无关个体的样本。观察到所有位点共 103 个等位基因。Hardy-Weinberg 平衡检验表明，在两个研究群体的 11 个 X-STR 位点中，所有位点的观察值均无显著偏离预期值（P>0.05）。各位点多态信息含量为 0.3864-0.9013，女性各位点的鉴别力为 0.6317-0.9845。11 个 X-STR 位点在藏族和北方汉族群体中的等位基因分布无统计学差异，与分子方差分析（AMOVA）结果一致。我们的工作表明，该多重系统可用于中国这两个群体的法医分析。
