Noroozi Zahra, Kido Horacio, Peytavi Régis, Nakajima-Sasaki Rie, Jasinskas Algimantas, Micic Miodrag, Felgner Philip L, Madou Marc J
Department of Mechanical and Aerospace Engineering, University of California, Irvine, 4200 Engineering Gateway, Irvine, California 92697-3975, USA.
Rev Sci Instrum. 2011 Jun;82(6):064303. doi: 10.1063/1.3597578.
A novel, centrifugal disk-based micro-total analysis system (μTAS) for low cost and high throughput semi-automated immunoassay processing was developed. A key innovation in the disposable immunoassay disk design is in a fluidic structure that enables very efficient micro-mixing based on a reciprocating mechanism in which centrifugal acceleration acting upon a liquid element first generates and stores pneumatic energy that is then released by a reduction of the centrifugal acceleration, resulting in a reversal of direction of flow of the liquid. Through an alternating sequence of high and low centrifugal acceleration, the system reciprocates the flow of liquid within the disk to maximize incubation/hybridization efficiency between antibodies and antigen macromolecules during the incubation/hybridization stage of the assay. The described reciprocating mechanism results in a reduction in processing time and reagent consumption by one order of magnitude.
开发了一种新型的基于离心盘的微全分析系统（μTAS），用于低成本、高通量的半自动免疫分析处理。一次性免疫分析盘设计的一项关键创新在于其流体结构，该结构基于一种往复机制实现了非常高效的微混合，在这种机制中，作用于液体元件的离心加速度首先产生并存储气动能量，然后通过降低离心加速度释放该能量，从而导致液体流动方向反转。通过高、低离心加速度的交替序列，该系统使盘内液体往复流动，以在分析的孵育/杂交阶段最大化抗体与抗原大分子之间的孵育/杂交效率。所描述的往复机制使处理时间和试剂消耗减少了一个数量级。
