一种基于往复式离心微流控技术的多重免疫分析系统。
A multiplexed immunoassay system based upon reciprocating centrifugal microfluidics.
作者信息
Noroozi Zahra, Kido Horacio, Peytavi Régis, Nakajima-Sasaki Rie, Jasinskas Algimantas, Micic Miodrag, Felgner Philip L, Madou Marc J
机构信息
Department of Mechanical and Aerospace Engineering, University of California, Irvine, 4200 Engineering Gateway, Irvine, California 92697-3975, USA.
出版信息
Rev Sci Instrum. 2011 Jun;82(6):064303. doi: 10.1063/1.3597578.
Abstract
A novel, centrifugal disk-based micro-total analysis system (μTAS) for low cost and high throughput semi-automated immunoassay processing was developed. A key innovation in the disposable immunoassay disk design is in a fluidic structure that enables very efficient micro-mixing based on a reciprocating mechanism in which centrifugal acceleration acting upon a liquid element first generates and stores pneumatic energy that is then released by a reduction of the centrifugal acceleration, resulting in a reversal of direction of flow of the liquid. Through an alternating sequence of high and low centrifugal acceleration, the system reciprocates the flow of liquid within the disk to maximize incubation/hybridization efficiency between antibodies and antigen macromolecules during the incubation/hybridization stage of the assay. The described reciprocating mechanism results in a reduction in processing time and reagent consumption by one order of magnitude.
摘要
开发了一种新型的基于离心盘的微全分析系统（μTAS），用于低成本、高通量的半自动免疫分析处理。一次性免疫分析盘设计的一项关键创新在于其流体结构，该结构基于一种往复机制实现了非常高效的微混合，在这种机制中，作用于液体元件的离心加速度首先产生并存储气动能量，然后通过降低离心加速度释放该能量，从而导致液体流动方向反转。通过高、低离心加速度的交替序列，该系统使盘内液体往复流动，以在分析的孵育/杂交阶段最大化抗体与抗原大分子之间的孵育/杂交效率。所描述的往复机制使处理时间和试剂消耗减少了一个数量级。
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本文引用的文献
1
Characterization of DNA hybridization kinetics in a microfluidic flow channel.微流控流动通道中DNA杂交动力学的表征
Sens Actuators B Chem. 2006 Jan 17;113(1):281-289. doi: 10.1016/j.snb.2005.03.034. Epub 2005 Apr 20.
2
A Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens.一种伯克霍尔德菌蛋白微阵列揭示了血清诊断抗原和交叉反应性抗原。
Proc Natl Acad Sci U S A. 2009 Aug 11;106(32):13499-504. doi: 10.1073/pnas.0812080106. Epub 2009 Jul 28.
3
Reciprocating flow-based centrifugal microfluidics mixer.基于往复流动的离心式微流控混合器。
Rev Sci Instrum. 2009 Jul;80(7):075102. doi: 10.1063/1.3169508.
4
A fully automated immunoassay from whole blood on a disc.一种基于圆盘的全血全自动免疫测定法。
Lab Chip. 2009 Jun 7;9(11):1548-55. doi: 10.1039/b820321k. Epub 2009 Mar 5.
5
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Electrophoresis. 2008 Apr;29(7):1399-406. doi: 10.1002/elps.200700898.
6
Improving an immunoassay response to related polychlorinated biphenyl analytes by mixing antibodies.
Anal Chem. 2006 Oct 15;78(20):7240-7. doi: 10.1021/ac0605187.
7
Protein and small molecule microarrays: powerful tools for high-throughput proteomics.蛋白质和小分子微阵列：高通量蛋白质组学的强大工具。
Mol Biosyst. 2006 Jan;2(1):58-68. doi: 10.1039/b513935j. Epub 2005 Nov 25.
8
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Annu Rev Biomed Eng. 2006;8:601-28. doi: 10.1146/annurev.bioeng.8.061505.095758.
9
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Bioinformatics. 2006 Jul 15;22(14):1760-6. doi: 10.1093/bioinformatics/btl162. Epub 2006 Apr 27.
10
Microfluidic device for rapid (<15 min) automated microarray hybridization.用于快速（<15分钟）自动微阵列杂交的微流控装置。
Clin Chem. 2005 Oct;51(10):1836-44. doi: 10.1373/clinchem.2005.052845. Epub 2005 Aug 17.
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