Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Physical Sciences, Dublin City University, Glasnevin, Dublin 9, Ireland.
Sensors (Basel). 2013 Aug 26;13(9):11336-49. doi: 10.3390/s130911336.
This paper demonstrates the full centrifugal microfluidic integration and automation of all liquid handling steps of a 7-step fluorescence-linked immunosorbent assay (FLISA) for quantifying nitrate and nitrite levels in whole blood within about 15 min. The assay protocol encompasses the extraction of metered plasma, the controlled release of sample and reagents (enzymes, co-factors and fluorescent labels), and incubation and detection steps. Flow control is implemented by a rotationally actuated dissolvable film (DF) valving scheme. In the valves, the burst pressure is primarily determined by the radial position, geometry and volume of the valve chamber and its inlet channel and can thus be individually tuned over an extraordinarily wide range of equivalent spin rates between 1,000 RPM and 5,500 RPM. Furthermore, the vapour barrier properties of the DF valves are investigated in this paper in order to further show the potential for commercially relevant on-board storage of liquid reagents during shelf-life of bioanalytical, ready-to-use discs.
本文展示了一种完整的离心微流控集成和自动化系统，可在大约 15 分钟内完成全血中硝酸盐和亚硝酸盐水平的 7 步荧光酶联免疫吸附测定（FLISA）的所有液体处理步骤。该测定方案包括定量血浆的提取、样品和试剂（酶、辅助因子和荧光标记物）的受控释放，以及孵育和检测步骤。流动控制通过旋转致动可溶解薄膜（DF）阀方案来实现。在阀中，破裂压力主要由阀室及其入口通道的径向位置、几何形状和体积决定，因此可以在 1000 RPM 至 5500 RPM 之间的非常宽的等效旋转速度范围内单独进行调整。此外，本文还研究了 DF 阀的蒸汽阻挡性能，以进一步展示在生物分析即用型圆盘的保质期内，对商业相关的板载液体试剂储存的潜在应用。
